Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases

László Poppe, Csaba Paizs, Klaudia Kovács, Florin Dan Irimie, Beáta Vértessy

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Citations (Scopus)


Ammonia-lyases catalyze a wide range of processes leading to α,β-unsaturated compounds by elimination of ammonia. In this chapter, ammonia-lyases are reviewed with major emphasis on their synthetic applications in stereoselective preparation of unnatural amino acids. Besides the synthesis of various unnatural α-amino acids with the aid of phenylalanine ammonia-lyases (PALs) utilizing the 3,5-dihydro-5-methylidene-4H-imidazol-4-one (MIO) prosthetic groups, the biotransformations leading to various unnatural β-amino acids with phenylalanine 2,3-aminomutases using the same catalytic MIO prosthetic group are discussed. Cloning, production, purification, and biotransformation protocols for PAL are described in detail.

Original languageEnglish
Title of host publicationUnnatural Amino Acids
Subtitle of host publicationMethods and Protocols
EditorsLoredano Pollegioni, Stefano Servi
Number of pages17
Publication statusPublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745



  • Biotransformation
  • Phenylalanine 2,3-aminomutase
  • Phenylalanine ammonia-lyase
  • Recombinant enzyme
  • Unnatural amino acid

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Poppe, L., Paizs, C., Kovács, K., Irimie, F. D., & Vértessy, B. (2012). Preparation of unnatural amino acids with ammonia-lyases and 2,3-aminomutases. In L. Pollegioni, & S. Servi (Eds.), Unnatural Amino Acids: Methods and Protocols (pp. 3-19). (Methods in Molecular Biology; Vol. 794).