Preparation of plants containing bacterial enzyme for degradation of polychlorinated biphenyls

Katerina Francova, Martina Sura, Tomas Macek, Miklos Szekeres, Simona Bancos, Katerina Demnerova, Michel Sylvestre, Martina Mackova

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The purpose of this investigation was to engineer genetically modified plants bearing the bacterial gene bphC coding for 2,3-dihydroxybiphenyl-1,2-dioxygenase. Gene bphC from Comamonas testosteroni B-356 was cloned into plasmid pBI 121 containing CaMV 35S promotor and kanamycine resistance and introduced into Agrobacterium tumefaciens LBA 4404. Plasmid pBI 121(B-356-bphC) was then transfered into Nicotiana tabacum L var. Wisconsin 38. Successful gene cloning in the plant was confirmed after transferring of the regenerants to selective medium by the root formation on rooting-supporting medium in the presence of kanamycine and by amplification of bphC from plant DNA using primers that are specific to the cloned gene.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalFresenius Environmental Bulletin
Volume12
Issue number3
Publication statusPublished - Jun 4 2003

    Fingerprint

Keywords

  • Degradation
  • Gene bphC
  • Phytoremediation
  • Polychlorinated biphenyls
  • Transgenic plants

ASJC Scopus subject areas

  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Francova, K., Sura, M., Macek, T., Szekeres, M., Bancos, S., Demnerova, K., Sylvestre, M., & Mackova, M. (2003). Preparation of plants containing bacterial enzyme for degradation of polychlorinated biphenyls. Fresenius Environmental Bulletin, 12(3), 309-313.