Preparation of plants containing bacterial enzyme for degradation of polychlorinated biphenyls

Katerina Francova, Martina Sura, Tomas Macek, M. Szekeres, Simona Bancos, Katerina Demnerova, Michel Sylvestre, Martina Mackova

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The purpose of this investigation was to engineer genetically modified plants bearing the bacterial gene bphC coding for 2,3-dihydroxybiphenyl-1,2-dioxygenase. Gene bphC from Comamonas testosteroni B-356 was cloned into plasmid pBI 121 containing CaMV 35S promotor and kanamycine resistance and introduced into Agrobacterium tumefaciens LBA 4404. Plasmid pBI 121(B-356-bphC) was then transfered into Nicotiana tabacum L var. Wisconsin 38. Successful gene cloning in the plant was confirmed after transferring of the regenerants to selective medium by the root formation on rooting-supporting medium in the presence of kanamycine and by amplification of bphC from plant DNA using primers that are specific to the cloned gene.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalFresenius Environmental Bulletin
Volume12
Issue number3
Publication statusPublished - 2003

Fingerprint

Plant Preparations
Polychlorinated Biphenyls
Polychlorinated biphenyls
PCB
Enzymes
Genes
enzyme
Degradation
degradation
gene
2,3-dihydroxybiphenyl oxygenase
plasmid
Genetically modified plants
Plasmids
Bearings (structural)
Plant DNA
DNA Primers
Cloning
rooting
Amplification

Keywords

  • Degradation
  • Gene bphC
  • Phytoremediation
  • Polychlorinated biphenyls
  • Transgenic plants

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Francova, K., Sura, M., Macek, T., Szekeres, M., Bancos, S., Demnerova, K., ... Mackova, M. (2003). Preparation of plants containing bacterial enzyme for degradation of polychlorinated biphenyls. Fresenius Environmental Bulletin, 12(3), 309-313.

Preparation of plants containing bacterial enzyme for degradation of polychlorinated biphenyls. / Francova, Katerina; Sura, Martina; Macek, Tomas; Szekeres, M.; Bancos, Simona; Demnerova, Katerina; Sylvestre, Michel; Mackova, Martina.

In: Fresenius Environmental Bulletin, Vol. 12, No. 3, 2003, p. 309-313.

Research output: Contribution to journalArticle

Francova, K, Sura, M, Macek, T, Szekeres, M, Bancos, S, Demnerova, K, Sylvestre, M & Mackova, M 2003, 'Preparation of plants containing bacterial enzyme for degradation of polychlorinated biphenyls', Fresenius Environmental Bulletin, vol. 12, no. 3, pp. 309-313.
Francova, Katerina ; Sura, Martina ; Macek, Tomas ; Szekeres, M. ; Bancos, Simona ; Demnerova, Katerina ; Sylvestre, Michel ; Mackova, Martina. / Preparation of plants containing bacterial enzyme for degradation of polychlorinated biphenyls. In: Fresenius Environmental Bulletin. 2003 ; Vol. 12, No. 3. pp. 309-313.
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