Preparation of Mono-125I-labelled gastrin-17 for radioimmunoassay measurements

J. Németh, M. Vecsernyés, G. Oroszi, Zs Balla, Z. Helyes, B. Farkas, Z. Szilvássy

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In the present work the efficiency of two oxidising agents (chloramine-T and iodogen) for human gastrin-17 labelling is investigated, whilst the purification of ion-exchange chromatography is compared with that of reversed-phase HPLC. In the Iodogen method the extent of radioactive iodine incorporation can be as high as 95-98% in contrast to 60-65% attained using the chloramine-T method. Reversed-phase HPLC purification proved to be superior to ion-exchange chromatography on the basis of a more distinct separation of mono-iodinated gastrin underlain by specific activity value (73 to 75 TBq/mmol) near identical with the theoretical one.

Original languageEnglish
Pages (from-to)855-863
Number of pages9
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume43
Issue number9
DOIs
Publication statusPublished - 2000

Fingerprint

Ion Exchange Chromatography
Chromatography
Radioimmunoassay
Purification
Ion exchange
High Pressure Liquid Chromatography
Gastrins
Oxidants
Iodine
Labeling
chloramine-T
1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril
gastrin 17

Keywords

  • Chloramine-T
  • HPLC
  • Human gastrin-17
  • Iodination
  • Iodogen
  • Ion-exchange chromatography

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Medicine
  • Analytical Chemistry
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Preparation of Mono-125I-labelled gastrin-17 for radioimmunoassay measurements. / Németh, J.; Vecsernyés, M.; Oroszi, G.; Balla, Zs; Helyes, Z.; Farkas, B.; Szilvássy, Z.

In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 43, No. 9, 2000, p. 855-863.

Research output: Contribution to journalArticle

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AU - Vecsernyés, M.

AU - Oroszi, G.

AU - Balla, Zs

AU - Helyes, Z.

AU - Farkas, B.

AU - Szilvássy, Z.

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