Preparation of highly purified human cytomegalovirus envelope antigen

F. Hudecz, E. Gönczöl, Stanley A. Plotkin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A human cytomegalovirus (HCMV) envelope preparation was highly purified by immunoaffinity column chromatography using an anti-cellular-IgG column. The purified envelope induced high titre antibodies to HCMV in guinea-pigs. Analysis of the guinea-pig immune sera by RIA and immunofluorescence (IF) showed that this envelope preparation, unlike its unpurified counterpar4 did not induce antibody to cellular contaminants. Dot-blot assay revealed viral proteins in the flow-through fraction and cellular proteins in the bound fractions. Results of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of flow-through and bound fractions suggest that a number of proteins previously identified as virus-specific may, in fac4 reflect cellular contamination of the envelope preparation, or crossreactivity of some virus-specific proteins with cellular proteins.

Original languageEnglish
Pages (from-to)300-304
Number of pages5
JournalVaccine
Volume3
Issue number4
DOIs
Publication statusPublished - 1985

Fingerprint

Human herpesvirus 5
Cytomegalovirus
antigens
Antigens
guinea pigs
Roseolovirus
Proteins
proteins
Viruses
viruses
antibodies
Antibodies
viral proteins
Viral Proteins
Sodium Dodecyl Sulfate
fluorescent antibody technique
Fluorescent Antibody Technique
polyacrylamide gel electrophoresis
antiserum
Chromatography

Keywords

  • HCMV envelope
  • Human cytomegalovirus
  • purification

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Preparation of highly purified human cytomegalovirus envelope antigen. / Hudecz, F.; Gönczöl, E.; Plotkin, Stanley A.

In: Vaccine, Vol. 3, No. 4, 1985, p. 300-304.

Research output: Contribution to journalArticle

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