Preparation, homogeneity and stability studies of a candidate LRM for Se speciation

Erzsébet Tünde Bodó, Z. Stefánka, I. Ipolyi, Csilla Sörös, M. Dernovics, P. Fodor

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A laboratory reference material (LRM) was prepared from Brazil nuts (Bertholletia excelsa) for quality control (QC) purposes of selenium speciation. The preparation of this LRM led through the usual operation steps applied during routine reference material production from biota samples-preparation of the raw material, homogenisation, storage design, checking of homogeneity, microbiological status and possible irradiation effects, and monitoring the species stability vs time at different storage temperatures. The selenium speciation studies to check species stability were carried out on a HPLC-UV-HG-AFS measurement set-up. Special attention was paid to the correct identification of selenium species by applying independent HPLC separation techniques (ion-pairing and anion-exchange chromatography). The concentration of selenomethionine (SeMet) and total Se content were quantified (79.9 μg g-1 (calculated as Se) and 82.9 μg g-1, respectively). The homogeneity and stability of this candidate reference material passed the relevant tests recommended by Bureau Communautaire de Référence (BCR).

Original languageEnglish
Pages (from-to)32-38
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume377
Issue number1
DOIs
Publication statusPublished - Sep 2003

Fingerprint

Bertholletia
Selenium
High Pressure Liquid Chromatography
Selenomethionine
Biota
Quality Control
Anions
Chromatography
Ions
Quality control
Ion exchange
Raw materials
Temperature
Irradiation
Monitoring

Keywords

  • Laboratory reference material
  • Selenium speciation
  • Selenomethionine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

Preparation, homogeneity and stability studies of a candidate LRM for Se speciation. / Bodó, Erzsébet Tünde; Stefánka, Z.; Ipolyi, I.; Sörös, Csilla; Dernovics, M.; Fodor, P.

In: Analytical and Bioanalytical Chemistry, Vol. 377, No. 1, 09.2003, p. 32-38.

Research output: Contribution to journalArticle

@article{b0dec9e1fa474d0d8e8b8e767ac9b532,
title = "Preparation, homogeneity and stability studies of a candidate LRM for Se speciation",
abstract = "A laboratory reference material (LRM) was prepared from Brazil nuts (Bertholletia excelsa) for quality control (QC) purposes of selenium speciation. The preparation of this LRM led through the usual operation steps applied during routine reference material production from biota samples-preparation of the raw material, homogenisation, storage design, checking of homogeneity, microbiological status and possible irradiation effects, and monitoring the species stability vs time at different storage temperatures. The selenium speciation studies to check species stability were carried out on a HPLC-UV-HG-AFS measurement set-up. Special attention was paid to the correct identification of selenium species by applying independent HPLC separation techniques (ion-pairing and anion-exchange chromatography). The concentration of selenomethionine (SeMet) and total Se content were quantified (79.9 μg g-1 (calculated as Se) and 82.9 μg g-1, respectively). The homogeneity and stability of this candidate reference material passed the relevant tests recommended by Bureau Communautaire de R{\'e}f{\'e}rence (BCR).",
keywords = "Laboratory reference material, Selenium speciation, Selenomethionine",
author = "Bod{\'o}, {Erzs{\'e}bet T{\"u}nde} and Z. Stef{\'a}nka and I. Ipolyi and Csilla S{\"o}r{\"o}s and M. Dernovics and P. Fodor",
year = "2003",
month = "9",
doi = "10.1007/s00216-003-1941-y",
language = "English",
volume = "377",
pages = "32--38",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Preparation, homogeneity and stability studies of a candidate LRM for Se speciation

AU - Bodó, Erzsébet Tünde

AU - Stefánka, Z.

AU - Ipolyi, I.

AU - Sörös, Csilla

AU - Dernovics, M.

AU - Fodor, P.

PY - 2003/9

Y1 - 2003/9

N2 - A laboratory reference material (LRM) was prepared from Brazil nuts (Bertholletia excelsa) for quality control (QC) purposes of selenium speciation. The preparation of this LRM led through the usual operation steps applied during routine reference material production from biota samples-preparation of the raw material, homogenisation, storage design, checking of homogeneity, microbiological status and possible irradiation effects, and monitoring the species stability vs time at different storage temperatures. The selenium speciation studies to check species stability were carried out on a HPLC-UV-HG-AFS measurement set-up. Special attention was paid to the correct identification of selenium species by applying independent HPLC separation techniques (ion-pairing and anion-exchange chromatography). The concentration of selenomethionine (SeMet) and total Se content were quantified (79.9 μg g-1 (calculated as Se) and 82.9 μg g-1, respectively). The homogeneity and stability of this candidate reference material passed the relevant tests recommended by Bureau Communautaire de Référence (BCR).

AB - A laboratory reference material (LRM) was prepared from Brazil nuts (Bertholletia excelsa) for quality control (QC) purposes of selenium speciation. The preparation of this LRM led through the usual operation steps applied during routine reference material production from biota samples-preparation of the raw material, homogenisation, storage design, checking of homogeneity, microbiological status and possible irradiation effects, and monitoring the species stability vs time at different storage temperatures. The selenium speciation studies to check species stability were carried out on a HPLC-UV-HG-AFS measurement set-up. Special attention was paid to the correct identification of selenium species by applying independent HPLC separation techniques (ion-pairing and anion-exchange chromatography). The concentration of selenomethionine (SeMet) and total Se content were quantified (79.9 μg g-1 (calculated as Se) and 82.9 μg g-1, respectively). The homogeneity and stability of this candidate reference material passed the relevant tests recommended by Bureau Communautaire de Référence (BCR).

KW - Laboratory reference material

KW - Selenium speciation

KW - Selenomethionine

UR - http://www.scopus.com/inward/record.url?scp=3042520300&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042520300&partnerID=8YFLogxK

U2 - 10.1007/s00216-003-1941-y

DO - 10.1007/s00216-003-1941-y

M3 - Article

C2 - 12750870

AN - SCOPUS:3042520300

VL - 377

SP - 32

EP - 38

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 1618-2642

IS - 1

ER -