Preparation, characterization, and potential application of an immobilized glucose oxidase

B. Szajáni, Aranka Molnár, Gabriella Klámar, M. Kálmán

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A simple, one-step process, using 0.25M p-benzoquinone dissolved in 20% dioxane at 50°C for 24 h was applied to the activation of polyacrylamide beads. The activated beads were reacted with glucose oxidase isolated from Aspergillus niger. The coupling reaction was performed in 0.1M potassium phosphate at pH 8.5 and 0-4°C for 24 h. The protein concentration was 50 mg/mL. In such conditions, the highest activity achieved was about 100 U/g solid. The optimum pH for the catalytic activity was shifted by about 1 pH unit in the acidic direction to pH 5.5. Between 35 and 50°C, the activity of the immobilized form depends on the temperature to a smaller extent than that of the soluble form. Above 50°C, the activity of immobilized glucose oxidase shows a sharper heat dependence. The enzyme-substrate interaction was not profoundly altered by the immobilization of the enzyme. The heat resistance of the immobilized enzyme was enhanced. The immobilized glucose oxidase is most stable at pH 5.5. The practical use of the immobilized glucose oxidase was tested in preliminary experiments for determination of the glucose concentration in blood sera.

Original languageEnglish
Pages (from-to)37-47
Number of pages11
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume14
Issue number1
DOIs
Publication statusPublished - Feb 1987

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Glucose Oxidase
Glucose oxidase
Enzymes
Immobilized Enzymes
Hot Temperature
Aspergillus
Polyacrylates
Heat resistance
Aspergillus niger
Glucose
Potassium
Catalyst activity
Phosphates
Blood
Immobilization
Chemical activation
Proteins
Substrates
Temperature
Serum

Keywords

  • catalytic properties, immobilized glucose oxidase
  • glucose determination, blood sera
  • Glucose oxidase, immobilized
  • polyacrylamidetype support, prepared by p-benzoquinon activation
  • support, polyacrylamide type

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Preparation, characterization, and potential application of an immobilized glucose oxidase. / Szajáni, B.; Molnár, Aranka; Klámar, Gabriella; Kálmán, M.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 14, No. 1, 02.1987, p. 37-47.

Research output: Contribution to journalArticle

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