Preparation, characterization and application of immobilized carboxypeptidase A

A. Vértesi, L. M. Simon, I. Kiss, B. Szajáni

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bovine carboxypeptidase A (peptidyl-L-amino-acid hydrolase, EC 3.4.17.1) was covalently attached to polyacrylamide beads and to silica-based supports, and also adsorbed on polyethylene terephthalate. The highest immobilized activity (125 U g-1 solid) was achieved when a polyacrylamide bead support (Akrilex C) possessing carboxylic functional groups activated by a water-soluble carbodiimide was used. The catalytic properties and stability of Akrilex C-carboxypeptidase A were studied and compared with the corresponding properties of the soluble enzyme. The optimum pH for the catalytic activity of the immobilized carboxypeptidase A was practically identical to that for the soluble enzyme (pH 7.5-8.0). The apparent optimum temperature of the immobilized carboxypeptidase A was about 20°C higher than that of the soluble enzyme. With hippuryl-L-phenylalanine as substrate, K(m app) for the immobilized enzyme (1.65 mM) was somewhat higher than K(m) for the soluble enzyme (1.07 mM). The conformational stability of the enzyme was markedly enhanced by the strongly hydrophilic microenvironment. The immobilized carboxypeptidase A was used for the C-terminal amino acid analysis of peptides. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalEnzyme and Microbial Technology
Volume25
Issue number1-2
DOIs
Publication statusPublished - Jul 15 1999

Fingerprint

Carboxypeptidases A
Enzymes
Polyacrylates
Amino acids
Carbodiimides
Amino Acids
Enzyme Stability
Immobilized Enzymes
Polyethylene Terephthalates
Hydrolases
Phenylalanine
Application programs
Silicon Dioxide
Functional groups
Catalyst activity
Polyethylene terephthalates
Peptides
Silica
Temperature
Water

Keywords

  • Carboxypeptidase A
  • Covalently immobilized
  • Immobilized carboxypeptidase A
  • Peptide digestion
  • Polyacrylamide type
  • Properties
  • Support

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Preparation, characterization and application of immobilized carboxypeptidase A. / Vértesi, A.; Simon, L. M.; Kiss, I.; Szajáni, B.

In: Enzyme and Microbial Technology, Vol. 25, No. 1-2, 15.07.1999, p. 73-79.

Research output: Contribution to journalArticle

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