Preparation and investigation of core-shell nanoparticles containing human interferon-α

Katalin Kristó, Márta Szekeres, Zsolt Makai, Árpád Márki, András Kelemen, László Bali, Zsolt Pallai, Imre Dékány, Ildikó Csóka

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Abstract

Sustained release of active interferon-α (IFN-α) has been achieved from core-shell nanoparticles (NPs) prepared by aqueous precipitation of IFN-α-enriched human serum albumin (HSA-IFN-α) and layer-by-layer (L-b-L) by coating of the IFN-α NPs with poly(sodium-4-styrene) sulphonate (PSS) and chitosan (Chit). The concentration and the pH of HSA solution were optimized during the development of this method. Dynamic light scattering (DLS), zeta-potential, thermal analysis (differential scanning calorimetry (DSC) and termogravimetry (TG)), X-ray diffraction (XRD), IFN-α activity and morphology (transmission electron microscope (TEM)) studies were used to control the preparation and analyse the products. The dissolution kinetics of NPs was measured in vitro over 7 days in Hanson dissolution tester with Millex membrane. In vivo studies in Pannon white rabbit detected steady IFN-α plasma level for 10 days after subcutaneous injection administration of the HSA-IFN-α NPs. The IFN-α plasma concentration was detected by using the enzyme-linked immunosorbent assay (ELISA) method. In the present paper we discuss the preparation method, the optimization steps and the results of in vitro and in vivo release studies. It was established that 76.13% HSA-IFN-α are encapsulated in the core-shell NPs.

Original languageEnglish
Article number118825
JournalInternational Journal of Pharmaceutics
Volume573
DOIs
Publication statusPublished - Jan 5 2020

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Keywords

  • Core-shell nanoparticle
  • Human serum albumin
  • In vivo release study
  • Interferon-α
  • Layer-by-layer self-assembly
  • Polyelectrolyte multilayers
  • Sustained release

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Kristó, K., Szekeres, M., Makai, Z., Márki, Á., Kelemen, A., Bali, L., Pallai, Z., Dékány, I., & Csóka, I. (2020). Preparation and investigation of core-shell nanoparticles containing human interferon-α. International Journal of Pharmaceutics, 573, [118825]. https://doi.org/10.1016/j.ijpharm.2019.118825