Preparation and investigation of bioactive transferrin-iron complexes formed with different synergistic anions

Judit Gálicza, Andrea Vargová, Viktor Sándor, Csongor Kálmán Orbán, Csaba Dezso András, Beáta Ábrahám, Szabolcs Lányi, Ferenc Kilár

Research output: Contribution to journalArticle

Abstract

Human serum transferrin has a potential for drug-delivery systems. Oxalate and aziridine-carboxylate was conjugated to serum transferrin in order to transport into the targeted cancer cells via transferrin-receptor mediated endocytosis. Capillary zone electrophoresis and capillary isoelectric focusing were used to analyze the effectiveness of complexation reactions. The electropherograms show the differences between iron-free- and ironcomplexed molecular forms of human serum transferrin. The iron-complexed transferrin sample containing the different anions as synergistic complexing agents were characterized by different electrophoretic parameters. Abstract Human serum transferrin has a potential for drug-delivery systems. Oxalate and aziridine-carboxylate was conjugated to serum transferrin in order to transport into the targeted cancer cells via transferrin-receptor mediated endocytosis. Capillary zone electrophoresis and capillary isoelectric focusing were used to analyze the effectiveness of complexation reactions. The electropherograms show the differences between iron-free- and ironcomplexed molecular forms of human serum transferrin. The iron-complexed transferrin sample containing the different anions as synergistic complexing agents were characterized by different electrophoretic parameters. Abstract Human serum transferrin has a potential for drug-delivery systems. Oxalate and aziridine-carboxylate was conjugated to serum transferrin in order to transport into the targeted cancer cells via transferrin-receptor mediated endocytosis. Capillary zone electrophoresis and capillary isoelectric focusing were used to analyze the effectiveness of complexation reactions. The electropherograms show the differences between iron-free- and ironcomplexed molecular forms of human serum transferrin. The iron-complexed transferrin sample containing the different anions as synergistic complexing agents were characterized by different electrophoretic parameters.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalProtein Journal
Volume31
Issue number1
DOIs
Publication statusPublished - Jan 1 2012

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Keywords

  • Aziridine-carboxylate
  • Capillary electrophoresis
  • Human serum transferrin
  • Oxalate
  • Targeted drug delivery

ASJC Scopus subject areas

  • Analytical Chemistry
  • Bioengineering
  • Biochemistry
  • Organic Chemistry

Cite this

Gálicza, J., Vargová, A., Sándor, V., Orbán, C. K., András, C. D., Ábrahám, B., Lányi, S., & Kilár, F. (2012). Preparation and investigation of bioactive transferrin-iron complexes formed with different synergistic anions. Protein Journal, 31(1), 27-34. https://doi.org/10.1007/s10930-011-9370-9