Preparation and investigation of a cross-linked hyaluronan nanoparticles system

Mónika Maroda, Magdolna Bodnár, Szilvia Berkó, József Bakó, Gábor Er̈s, Erzsébet Csányi, P. Szabó-Révész, John F. Hartmann, L. Kemény, J. Borbély

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The present paper describes the preparation and characterization of hydrophilic hyaluronan nanoparticles formed by cross-linking of hyaluronic acid (HA) with 2,2′(ethylenedioxy)bis(ethylamine) in the presence of water-soluble carbodiimide (CDI) in aqueous media. The particle size of cross-linked nanosystems was measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM), transmittance by UV-VIS spectrophotometry, gel permeation chromatography (GPC) and rheology to characterize the physico-chemical properties. The aqueous nanosystems prepared were stable, transparent or mildly opalescent with values of transmittance above 91%. It was observed, that a fraction of particles with size less than 20 nm, has been released during the purification by diafiltration. It was established that the hydrodynamic size of cross-linked HA nanoparticles can be controlled by variation of the reaction conditions such as concentration of HA, salt concentration of media, ratio of cross-linker and the final pH of the reaction mixture.

Original languageEnglish
Pages (from-to)1322-1329
Number of pages8
JournalCarbohydrate Polymers
Volume83
Issue number3
DOIs
Publication statusPublished - Jan 30 2011

Fingerprint

Hyaluronic acid
Hyaluronic Acid
Nanosystems
Nanoparticles
Spectrophotometry
Gel permeation chromatography
Dynamic light scattering
Light transmission
Rheology
Carbodiimides
Chemical properties
Purification
Hydrodynamics
Particle size
Salts
Transmission electron microscopy
Water

Keywords

  • Cross-linking
  • Hyaluronic acid
  • Nanoparticle
  • Nanosphere

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics

Cite this

Preparation and investigation of a cross-linked hyaluronan nanoparticles system. / Maroda, Mónika; Bodnár, Magdolna; Berkó, Szilvia; Bakó, József; Er̈s, Gábor; Csányi, Erzsébet; Szabó-Révész, P.; Hartmann, John F.; Kemény, L.; Borbély, J.

In: Carbohydrate Polymers, Vol. 83, No. 3, 30.01.2011, p. 1322-1329.

Research output: Contribution to journalArticle

Maroda M, Bodnár M, Berkó S, Bakó J, Er̈s G, Csányi E et al. Preparation and investigation of a cross-linked hyaluronan nanoparticles system. Carbohydrate Polymers. 2011 Jan 30;83(3):1322-1329. https://doi.org/10.1016/j.carbpol.2010.09.039
Maroda, Mónika ; Bodnár, Magdolna ; Berkó, Szilvia ; Bakó, József ; Er̈s, Gábor ; Csányi, Erzsébet ; Szabó-Révész, P. ; Hartmann, John F. ; Kemény, L. ; Borbély, J. / Preparation and investigation of a cross-linked hyaluronan nanoparticles system. In: Carbohydrate Polymers. 2011 ; Vol. 83, No. 3. pp. 1322-1329.
@article{df51fd9e37494341ac819f81cefacf61,
title = "Preparation and investigation of a cross-linked hyaluronan nanoparticles system",
abstract = "The present paper describes the preparation and characterization of hydrophilic hyaluronan nanoparticles formed by cross-linking of hyaluronic acid (HA) with 2,2′(ethylenedioxy)bis(ethylamine) in the presence of water-soluble carbodiimide (CDI) in aqueous media. The particle size of cross-linked nanosystems was measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM), transmittance by UV-VIS spectrophotometry, gel permeation chromatography (GPC) and rheology to characterize the physico-chemical properties. The aqueous nanosystems prepared were stable, transparent or mildly opalescent with values of transmittance above 91{\%}. It was observed, that a fraction of particles with size less than 20 nm, has been released during the purification by diafiltration. It was established that the hydrodynamic size of cross-linked HA nanoparticles can be controlled by variation of the reaction conditions such as concentration of HA, salt concentration of media, ratio of cross-linker and the final pH of the reaction mixture.",
keywords = "Cross-linking, Hyaluronic acid, Nanoparticle, Nanosphere",
author = "M{\'o}nika Maroda and Magdolna Bodn{\'a}r and Szilvia Berk{\'o} and J{\'o}zsef Bak{\'o} and G{\'a}bor Er̈s and Erzs{\'e}bet Cs{\'a}nyi and P. Szab{\'o}-R{\'e}v{\'e}sz and Hartmann, {John F.} and L. Kem{\'e}ny and J. Borb{\'e}ly",
year = "2011",
month = "1",
day = "30",
doi = "10.1016/j.carbpol.2010.09.039",
language = "English",
volume = "83",
pages = "1322--1329",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Preparation and investigation of a cross-linked hyaluronan nanoparticles system

AU - Maroda, Mónika

AU - Bodnár, Magdolna

AU - Berkó, Szilvia

AU - Bakó, József

AU - Er̈s, Gábor

AU - Csányi, Erzsébet

AU - Szabó-Révész, P.

AU - Hartmann, John F.

AU - Kemény, L.

AU - Borbély, J.

PY - 2011/1/30

Y1 - 2011/1/30

N2 - The present paper describes the preparation and characterization of hydrophilic hyaluronan nanoparticles formed by cross-linking of hyaluronic acid (HA) with 2,2′(ethylenedioxy)bis(ethylamine) in the presence of water-soluble carbodiimide (CDI) in aqueous media. The particle size of cross-linked nanosystems was measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM), transmittance by UV-VIS spectrophotometry, gel permeation chromatography (GPC) and rheology to characterize the physico-chemical properties. The aqueous nanosystems prepared were stable, transparent or mildly opalescent with values of transmittance above 91%. It was observed, that a fraction of particles with size less than 20 nm, has been released during the purification by diafiltration. It was established that the hydrodynamic size of cross-linked HA nanoparticles can be controlled by variation of the reaction conditions such as concentration of HA, salt concentration of media, ratio of cross-linker and the final pH of the reaction mixture.

AB - The present paper describes the preparation and characterization of hydrophilic hyaluronan nanoparticles formed by cross-linking of hyaluronic acid (HA) with 2,2′(ethylenedioxy)bis(ethylamine) in the presence of water-soluble carbodiimide (CDI) in aqueous media. The particle size of cross-linked nanosystems was measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM), transmittance by UV-VIS spectrophotometry, gel permeation chromatography (GPC) and rheology to characterize the physico-chemical properties. The aqueous nanosystems prepared were stable, transparent or mildly opalescent with values of transmittance above 91%. It was observed, that a fraction of particles with size less than 20 nm, has been released during the purification by diafiltration. It was established that the hydrodynamic size of cross-linked HA nanoparticles can be controlled by variation of the reaction conditions such as concentration of HA, salt concentration of media, ratio of cross-linker and the final pH of the reaction mixture.

KW - Cross-linking

KW - Hyaluronic acid

KW - Nanoparticle

KW - Nanosphere

UR - http://www.scopus.com/inward/record.url?scp=78650281255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650281255&partnerID=8YFLogxK

U2 - 10.1016/j.carbpol.2010.09.039

DO - 10.1016/j.carbpol.2010.09.039

M3 - Article

AN - SCOPUS:78650281255

VL - 83

SP - 1322

EP - 1329

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

IS - 3

ER -