Preparation and characterization of immobilized glucose-phosphate isomerase

L. M. Simon, M. Kotormán, B. Szajáni, L. Boross

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Glucose-phosphate isomerase (d-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) isolated from rabbit muscle was covalently attached to Sepharose 4B, Akrilex C-100, Akrilex P-100 and Silochrome aldehyde, respectively. The highest immobilized activity (110 units mg-1solid) was achieved when the enzyme was bound to Akrilex C-100, a polyacrylamide support possessing carboxylic functional groups. The catalytic properties and the stability of this Akrilex-bound enzyme were studied in detail relative to those of the soluble enzyme. For both soluble and immobilized glucose-phosphate isomerase the pH optimum was about pH 8.0. The apparent optimum temperature of the immobilized enzyme was about 50°C, while that for the soluble enzyme lay between 50 and 55°C. The apparent Km for glucose 6-phosphate as substrate was higher (2 × 10-1 mM) in the case of the immobilized enzyme. Little difference was found between the thermal stabilities of the soluble enzyme (at 45°C t 1 2 = 42 min; at 50°C t 1 2 = 12 min) and the immobilized enzyme (at 45°C t 1 2 = 82 min; at 50°C t 1 2 = 10 min). As a result of immobilization, the stability against urea was increased.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalEnzyme and Microbial Technology
Volume8
Issue number4
DOIs
Publication statusPublished - 1986

Fingerprint

xylose isomerase
Isomerases
Immobilized Enzymes
Glucose
Phosphates
Enzymes
Glucose-6-Phosphate
Glucose-6-Phosphate Isomerase
Enzyme Stability
Silica Gel
Aldehydes
Immobilization
Sepharose
Urea
Hot Temperature
Functional groups
Muscle
Rabbits
Thermodynamic stability
Muscles

Keywords

  • covalent attachment, catalytic properties, stability tests
  • Immobilized glucose-phosphate isomerase

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Preparation and characterization of immobilized glucose-phosphate isomerase. / Simon, L. M.; Kotormán, M.; Szajáni, B.; Boross, L.

In: Enzyme and Microbial Technology, Vol. 8, No. 4, 1986, p. 222-226.

Research output: Contribution to journalArticle

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