Preparation and characterization of cationic pluronic for surface modification and functionalization of polymeric drug delivery nanoparticles

G. Gyulai, A. Magyar, J. Rohonczy, J. Orosz, M. Yamasaki, Sz. Bősze, E. Kiss

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Biodegradable poly(lactic-co-glycolic acid) copolymer, PLGA nanoparticles (NPs) with a surface layer of poly (ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymers, Pluronics, are promising drug carrier systems. With the aim to increase the potential of targeted drug delivery the end group derivative of Pluronics was synthesized in a straightforward way to obtain Pluronic-amines. The formation of functional amine groups was confirmed by fluorescamine method and NMR analysis of their N-(tert-Butoxycarbonyl)-L-phenylalanine (Boc-Phe-OH) and N-(9-Fluorenylmethoxycarbonyl)- L-phenylalanine (Fmoc-Phe-OH) conjugates. Pluronic and Pluronic-amine stabilized PLGA NPs prepared by nanoprecipitation were characterized by dynamic light scattering and zeta potential measurements. All of the systems showed high colloidal stability checked by electrolyte induced aggregation, although the presence of Pluronicamine on the surface decreased the zeta potential in some extent. The introduction of reactive primary amine groups into the surface layer of PLGA NPs while preserving the aggregation stability, provides a possibility for coupling of various ligands allowing targeted delivery and also contributes to the improved membrane affinity of NPs.

Original languageEnglish
Pages (from-to)216-226
Number of pages11
JournalExpress Polymer Letters
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Fingerprint

Poloxamer
Drug delivery
Surface treatment
Amines
delivery
amines
drugs
Nanoparticles
nanoparticles
preparation
phenylalanine
Zeta potential
ethylene oxide
Polyethylene oxides
surface layers
copolymers
Phenylalanine
Agglomeration
Polypropylene oxides
propylene oxide

Keywords

  • Biocompatible polymers
  • Colloidal stability
  • PLGA nanoparticles
  • Pluronic derivatization
  • Polymeric drug delivery

ASJC Scopus subject areas

  • Polymers and Plastics
  • Materials Chemistry
  • Chemical Engineering(all)
  • Organic Chemistry
  • Physical and Theoretical Chemistry

Cite this

Preparation and characterization of cationic pluronic for surface modification and functionalization of polymeric drug delivery nanoparticles. / Gyulai, G.; Magyar, A.; Rohonczy, J.; Orosz, J.; Yamasaki, M.; Bősze, Sz.; Kiss, E.

In: Express Polymer Letters, Vol. 10, No. 3, 01.03.2016, p. 216-226.

Research output: Contribution to journalArticle

@article{207cf940c52742b19ab16bd9e737e420,
title = "Preparation and characterization of cationic pluronic for surface modification and functionalization of polymeric drug delivery nanoparticles",
abstract = "Biodegradable poly(lactic-co-glycolic acid) copolymer, PLGA nanoparticles (NPs) with a surface layer of poly (ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymers, Pluronics, are promising drug carrier systems. With the aim to increase the potential of targeted drug delivery the end group derivative of Pluronics was synthesized in a straightforward way to obtain Pluronic-amines. The formation of functional amine groups was confirmed by fluorescamine method and NMR analysis of their N-(tert-Butoxycarbonyl)-L-phenylalanine (Boc-Phe-OH) and N-(9-Fluorenylmethoxycarbonyl)- L-phenylalanine (Fmoc-Phe-OH) conjugates. Pluronic and Pluronic-amine stabilized PLGA NPs prepared by nanoprecipitation were characterized by dynamic light scattering and zeta potential measurements. All of the systems showed high colloidal stability checked by electrolyte induced aggregation, although the presence of Pluronicamine on the surface decreased the zeta potential in some extent. The introduction of reactive primary amine groups into the surface layer of PLGA NPs while preserving the aggregation stability, provides a possibility for coupling of various ligands allowing targeted delivery and also contributes to the improved membrane affinity of NPs.",
keywords = "Biocompatible polymers, Colloidal stability, PLGA nanoparticles, Pluronic derivatization, Polymeric drug delivery",
author = "G. Gyulai and A. Magyar and J. Rohonczy and J. Orosz and M. Yamasaki and Sz. Bősze and E. Kiss",
year = "2016",
month = "3",
day = "1",
doi = "10.3144/expresspolymlett.2016.20",
language = "English",
volume = "10",
pages = "216--226",
journal = "Express Polymer Letters",
issn = "1788-618X",
publisher = "BME-PT and GTE",
number = "3",

}

TY - JOUR

T1 - Preparation and characterization of cationic pluronic for surface modification and functionalization of polymeric drug delivery nanoparticles

AU - Gyulai, G.

AU - Magyar, A.

AU - Rohonczy, J.

AU - Orosz, J.

AU - Yamasaki, M.

AU - Bősze, Sz.

AU - Kiss, E.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Biodegradable poly(lactic-co-glycolic acid) copolymer, PLGA nanoparticles (NPs) with a surface layer of poly (ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymers, Pluronics, are promising drug carrier systems. With the aim to increase the potential of targeted drug delivery the end group derivative of Pluronics was synthesized in a straightforward way to obtain Pluronic-amines. The formation of functional amine groups was confirmed by fluorescamine method and NMR analysis of their N-(tert-Butoxycarbonyl)-L-phenylalanine (Boc-Phe-OH) and N-(9-Fluorenylmethoxycarbonyl)- L-phenylalanine (Fmoc-Phe-OH) conjugates. Pluronic and Pluronic-amine stabilized PLGA NPs prepared by nanoprecipitation were characterized by dynamic light scattering and zeta potential measurements. All of the systems showed high colloidal stability checked by electrolyte induced aggregation, although the presence of Pluronicamine on the surface decreased the zeta potential in some extent. The introduction of reactive primary amine groups into the surface layer of PLGA NPs while preserving the aggregation stability, provides a possibility for coupling of various ligands allowing targeted delivery and also contributes to the improved membrane affinity of NPs.

AB - Biodegradable poly(lactic-co-glycolic acid) copolymer, PLGA nanoparticles (NPs) with a surface layer of poly (ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymers, Pluronics, are promising drug carrier systems. With the aim to increase the potential of targeted drug delivery the end group derivative of Pluronics was synthesized in a straightforward way to obtain Pluronic-amines. The formation of functional amine groups was confirmed by fluorescamine method and NMR analysis of their N-(tert-Butoxycarbonyl)-L-phenylalanine (Boc-Phe-OH) and N-(9-Fluorenylmethoxycarbonyl)- L-phenylalanine (Fmoc-Phe-OH) conjugates. Pluronic and Pluronic-amine stabilized PLGA NPs prepared by nanoprecipitation were characterized by dynamic light scattering and zeta potential measurements. All of the systems showed high colloidal stability checked by electrolyte induced aggregation, although the presence of Pluronicamine on the surface decreased the zeta potential in some extent. The introduction of reactive primary amine groups into the surface layer of PLGA NPs while preserving the aggregation stability, provides a possibility for coupling of various ligands allowing targeted delivery and also contributes to the improved membrane affinity of NPs.

KW - Biocompatible polymers

KW - Colloidal stability

KW - PLGA nanoparticles

KW - Pluronic derivatization

KW - Polymeric drug delivery

UR - http://www.scopus.com/inward/record.url?scp=84953455708&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84953455708&partnerID=8YFLogxK

U2 - 10.3144/expresspolymlett.2016.20

DO - 10.3144/expresspolymlett.2016.20

M3 - Article

AN - SCOPUS:84953455708

VL - 10

SP - 216

EP - 226

JO - Express Polymer Letters

JF - Express Polymer Letters

SN - 1788-618X

IS - 3

ER -