Preparation and a simple one-step purification of [His1-mono-125I-Tyr10,Nle27] hGHRH(1-32)-NH2

J. Gardi, James M. Krueger, Robert C. Speth

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A one-step purification of [His1-mono-125I-Tyr10,Nle27] -hGHRH(1-32)-NH2, prepared using chloramine-T, by HPLC with isocratic elution is described. The labeled GHRH analog was suitable for GHRH receptor binding assays.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume45
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Purification
Assays
High Pressure Liquid Chromatography
chloramine-T
somatotropin releasing hormone receptor

Keywords

  • Binding assay
  • HPLC
  • Isocratic
  • Mono-I-GHRH analog

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Organic Chemistry
  • Clinical Biochemistry
  • Molecular Medicine
  • Pharmacology

Cite this

Preparation and a simple one-step purification of [His1-mono-125I-Tyr10,Nle27] hGHRH(1-32)-NH2. / Gardi, J.; Krueger, James M.; Speth, Robert C.

In: Journal of Labelled Compounds and Radiopharmaceuticals, Vol. 45, No. 1, 2002, p. 13-18.

Research output: Contribution to journalArticle

@article{bdf3ba9e5058475aa61171c41891e257,
title = "Preparation and a simple one-step purification of [His1-mono-125I-Tyr10,Nle27] hGHRH(1-32)-NH2",
abstract = "A one-step purification of [His1-mono-125I-Tyr10,Nle27] -hGHRH(1-32)-NH2, prepared using chloramine-T, by HPLC with isocratic elution is described. The labeled GHRH analog was suitable for GHRH receptor binding assays.",
keywords = "Binding assay, HPLC, Isocratic, Mono-I-GHRH analog",
author = "J. Gardi and Krueger, {James M.} and Speth, {Robert C.}",
year = "2002",
doi = "10.1002/jlcr.526",
language = "English",
volume = "45",
pages = "13--18",
journal = "Journal of Labelled Compounds and Radiopharmaceuticals",
issn = "0362-4803",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Preparation and a simple one-step purification of [His1-mono-125I-Tyr10,Nle27] hGHRH(1-32)-NH2

AU - Gardi, J.

AU - Krueger, James M.

AU - Speth, Robert C.

PY - 2002

Y1 - 2002

N2 - A one-step purification of [His1-mono-125I-Tyr10,Nle27] -hGHRH(1-32)-NH2, prepared using chloramine-T, by HPLC with isocratic elution is described. The labeled GHRH analog was suitable for GHRH receptor binding assays.

AB - A one-step purification of [His1-mono-125I-Tyr10,Nle27] -hGHRH(1-32)-NH2, prepared using chloramine-T, by HPLC with isocratic elution is described. The labeled GHRH analog was suitable for GHRH receptor binding assays.

KW - Binding assay

KW - HPLC

KW - Isocratic

KW - Mono-I-GHRH analog

UR - http://www.scopus.com/inward/record.url?scp=0036151054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036151054&partnerID=8YFLogxK

U2 - 10.1002/jlcr.526

DO - 10.1002/jlcr.526

M3 - Article

VL - 45

SP - 13

EP - 18

JO - Journal of Labelled Compounds and Radiopharmaceuticals

JF - Journal of Labelled Compounds and Radiopharmaceuticals

SN - 0362-4803

IS - 1

ER -