Preoptic LH-RH and somatostatin in the rat median eminence: An experimental light and electron microscopic immunocytochemical study

Jean Y. Jew, Csaba Leränth, Akira Arimura, M. Palkóvits

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Glass microknife lesions and immunocytochemistry were used to evaluate luteinizing hormone-releasing hormone (LH-RH)- and somatostatin (SS)-immunoreactive pathways from the preoptic region to the rat median eminence. Cuts were so placed that axons of more caudally located neurons in the periventricular hypothalamic areas were spared. Light and EM observations of LH-RH-immunostained preparations indicated that following the midline periventricular cuts the density of LH-RH labelled axons and axon terminals in the ME appeared similar to that of nonlesioned animals. Following bilateral lateral hypothalamic cuts placed between the preoptic area and the ME, LH-RH immunostain- ing in the ME was markedly reduced. This provides evidence that the preponderance of LH-RH axons originating from the preoptic area reach the ME by a lateral hypothalamic route. In contrast to the LH-RH findings, midline lesions made using the same coordinates caused a noticeable reduction in SS immunostaining in the arcuate nucleus and ME. There was either no change or only minimal change after the lateral cut. Somatostatin axons arising from the preoptic periventricular nucleus take a periventricular route and contribute to median eminence innervation, but much less extensively than the more caudally located somatostatin neurons in the hypothalamic periventricular nucleus [19].

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalNeuroendocrinology
Volume38
Issue number3
Publication statusPublished - 1984

Fingerprint

Median Eminence
Somatostatin
Gonadotropin-Releasing Hormone
Electrons
Light
Preoptic Area
Axons
Neurons
Arcuate Nucleus of Hypothalamus
Presynaptic Terminals
Glass
Immunohistochemistry

Keywords

  • Arcuate nucleus
  • Immuno electron microscopy
  • LH-RH
  • Median eminence
  • Somatostatin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Preoptic LH-RH and somatostatin in the rat median eminence : An experimental light and electron microscopic immunocytochemical study. / Jew, Jean Y.; Leränth, Csaba; Arimura, Akira; Palkóvits, M.

In: Neuroendocrinology, Vol. 38, No. 3, 1984, p. 169-175.

Research output: Contribution to journalArticle

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