Prenatal diagnosis of cystic fibrosis by microvillar membrane enzyme analysis in amniotic fluid.

M. Szabó, F. Teichmann, M. Huszka, A. Münnich, L. Veress, Z. Papp

Research output: Contribution to journalArticle

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Abstract

Prenatal diagnosis was performed in 92 pregnancies high-risk for cystic fibrosis during six years. Amniotic fluid samples obtained by amniocentesis were examined with regard to their microvillar membrane enzyme activity. Though trehalase, alkaline phosphatase isoenzymes and L-gamma-glutamyltransferase in the amniotic fluid are not specific markers of cystic fibrosis, their activity is significantly lower than in normal pregnancies. By measuring the three enzymes simultaneously, sensitivity, specificity and reliability of the method were found to be over 92%. It is concluded that mid-trimester amniotic fluid diagnosis is indispensable for some heterozygotic couples for cystic fibrosis even in the possession of DNA (desoxyrobonucleic acid) methods.

Original languageEnglish
Pages (from-to)263-274
Number of pages12
JournalActa Paediatrica Hungarica
Volume31
Issue number2
Publication statusPublished - 1991

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Amniotic Fluid
Prenatal Diagnosis
Cystic Fibrosis
Membranes
Enzymes
Trehalase
High-Risk Pregnancy
Amniocentesis
gamma-Glutamyltransferase
Isoenzymes
Alkaline Phosphatase
Sensitivity and Specificity
Pregnancy
Acids

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Szabó, M., Teichmann, F., Huszka, M., Münnich, A., Veress, L., & Papp, Z. (1991). Prenatal diagnosis of cystic fibrosis by microvillar membrane enzyme analysis in amniotic fluid. Acta Paediatrica Hungarica, 31(2), 263-274.

Prenatal diagnosis of cystic fibrosis by microvillar membrane enzyme analysis in amniotic fluid. / Szabó, M.; Teichmann, F.; Huszka, M.; Münnich, A.; Veress, L.; Papp, Z.

In: Acta Paediatrica Hungarica, Vol. 31, No. 2, 1991, p. 263-274.

Research output: Contribution to journalArticle

Szabó, M, Teichmann, F, Huszka, M, Münnich, A, Veress, L & Papp, Z 1991, 'Prenatal diagnosis of cystic fibrosis by microvillar membrane enzyme analysis in amniotic fluid.', Acta Paediatrica Hungarica, vol. 31, no. 2, pp. 263-274.
Szabó, M. ; Teichmann, F. ; Huszka, M. ; Münnich, A. ; Veress, L. ; Papp, Z. / Prenatal diagnosis of cystic fibrosis by microvillar membrane enzyme analysis in amniotic fluid. In: Acta Paediatrica Hungarica. 1991 ; Vol. 31, No. 2. pp. 263-274.
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