Preliminary results of layer chromatography combined with bilateral band compression to search active minor components

Ágnes M. Móricz, Emil Mincsovics, Péter G. Ott

Research output: Contribution to journalArticle


Bilateral band compression (BBC) is a process where a solvent flow perpendicular to the direction of development squeezes bands into a smaller area. BBC of the 10 mm wide lanes of high-performance thin-layer chromatography (HPTLC) separation resulted in more than 6 times increase in peak height and peak area of Canadian goldenrod labdane diterpenes solidagenone and presolidagenone. Simultaneously, the signal-to-noise ratio increased only up to 2.2–2.4 times due to the higher noise level. The BBC increased the relative standard deviations (RSDs) of peak height and area reached three to four times of the original as well. It is known that overpressured layer chromatography (OPLC) separates more efficiently than conventional TLC/HPTLC techniques. In order to increase the bio-detection sensitivity, we combined bioautography, BBC, and fully offline infusion-OPLC using band-shaped sample application/separation. Thyme essential oil has been applied as a multi-component object to show the potential of this process. It is demonstrated that biologically active minor components, which may be missed in case of a conventional process, can also be detected by means of this combined method.

Original languageEnglish
JournalJournal of Liquid Chromatography and Related Technologies
Publication statusAccepted/In press - Jan 1 2020



  • Bilateral band compression
  • Canadian goldenrod (Solidago canadensis L.) root extract
  • direct bioautography
  • high-performance thin-layer chromatography (HPTLC)
  • increased sensitivity
  • infusion overpressured layer chromatography (OPLC)
  • thyme essential oil

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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