Preformed postribosomal pool of Fc receptors on human peripheral mononuclear blood cells

K. Liszka, J. Schrett, J. Gergely

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The shedding of surface structures due to a 4°C to 37°C temperature shift is followed by a regeneration within 4 h. To demonstrate the shedding and reappearance of Fc receptors, the method of anti-D EA rosettes was used. Three subsequent temperature shifts were followed by repeated reappearance of FcR in 76% of the cases investigated. In several cases, however, besides the shedding of FcR into the medium, the percentage of Ea rosette-forming cells did not decrease, which points to the possibility that repeated temperature shifts may cause shedding and expression of FcR at the same time. In 24% of the cases we could not observe repeated reappearances, possibly due to the exhaustion of an FcR reservoir. Inhibition of protein synthesis with cycloheximide, suppression of cell metabolism by adjustment to low temperatures and addition of sodium azide did not influence the reappearance of FcR following the temperature shift. These findings suggest that the reappearance of FcR after temperature shift is the consequence of a membrane rearrangement in which preformed FcR become expressed on the cell surface.

Original languageEnglish
Pages (from-to)129-132
Number of pages4
JournalImmunology Letters
Volume1
Issue number3
DOIs
Publication statusPublished - 1979

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Fc Receptors
Blood Cells
Temperature
Sodium Azide
Cycloheximide
Regeneration
Membranes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Preformed postribosomal pool of Fc receptors on human peripheral mononuclear blood cells. / Liszka, K.; Schrett, J.; Gergely, J.

In: Immunology Letters, Vol. 1, No. 3, 1979, p. 129-132.

Research output: Contribution to journalArticle

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