Preferential utilisation of deoxycytidine by undifferentiated (peanut positive) tonsillar lymphocytes

J. Taljanidisz, Tatjana Spasokukotskaja, M. Sasvári, F. Antoni, M. Staub

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Peanut agglutinin (PNA), a d-galactose specific lectin, agglutinated 10-18% of lymphocytes isolated from tonsils of 3- to 6-yr-old children. PNA+ cells were found to be mainly B lymphocytes showing a 9.6 times higher specific activity of DNA polymerase alpha compared to the PNA- cells. The specific activity of deoxycytidine kinase as well as the incorporation of [5-3H]deoxycytidine were also much higher in PNA+ cell fraction than in PNA- fraction (7.7-fold and 6-fold, respectively). On the other hand, thymidine kinase activity and [5-3H]deoxythymidine incorporation were only 3.6 and 3.9 times higher, respectively. The data presented here show a high degree of DNA synthesis and preferential utilisation of [5-3H]deoxycytidine for DNA synthesis in this undifferentiated B lymphocyte fraction.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalImmunology Letters
Volume15
Issue number2
DOIs
Publication statusPublished - 1987

Fingerprint

Peanut Agglutinin
Deoxycytidine
Lymphocytes
B-Lymphocytes
Deoxycytidine Kinase
DNA Polymerase I
Thymidine Kinase
Palatine Tonsil
DNA
Galactose
Lectins
Arachis

Keywords

  • Deoxycytidine kinase
  • Differentiation
  • Lymphocyte
  • Salvage
  • Thymidine kinase

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Preferential utilisation of deoxycytidine by undifferentiated (peanut positive) tonsillar lymphocytes. / Taljanidisz, J.; Spasokukotskaja, Tatjana; Sasvári, M.; Antoni, F.; Staub, M.

In: Immunology Letters, Vol. 15, No. 2, 1987, p. 109-115.

Research output: Contribution to journalArticle

Taljanidisz, J. ; Spasokukotskaja, Tatjana ; Sasvári, M. ; Antoni, F. ; Staub, M. / Preferential utilisation of deoxycytidine by undifferentiated (peanut positive) tonsillar lymphocytes. In: Immunology Letters. 1987 ; Vol. 15, No. 2. pp. 109-115.
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AU - Antoni, F.

AU - Staub, M.

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