Preferential utilisation of deoxycytidine by undifferentiated (peanut positive) tonsillar lymphocytes

J. Taljanidisz, Tatjana Spasokukotskaja, Mária Sasvári-Székely, F. Antoni, Mária Staub

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7 Citations (Scopus)

Abstract

Peanut agglutinin (PNA), a d-galactose specific lectin, agglutinated 10-18% of lymphocytes isolated from tonsils of 3- to 6-yr-old children. PNA+ cells were found to be mainly B lymphocytes showing a 9.6 times higher specific activity of DNA polymerase alpha compared to the PNA- cells. The specific activity of deoxycytidine kinase as well as the incorporation of [5-3H]deoxycytidine were also much higher in PNA+ cell fraction than in PNA- fraction (7.7-fold and 6-fold, respectively). On the other hand, thymidine kinase activity and [5-3H]deoxythymidine incorporation were only 3.6 and 3.9 times higher, respectively. The data presented here show a high degree of DNA synthesis and preferential utilisation of [5-3H]deoxycytidine for DNA synthesis in this undifferentiated B lymphocyte fraction.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalImmunology letters
Volume15
Issue number2
DOIs
Publication statusPublished - Jun 1987

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Keywords

  • Deoxycytidine kinase
  • Differentiation
  • Lymphocyte
  • Salvage
  • Thymidine kinase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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