Preferential transport of glutathione versus glutathione disulfide in rat liver microsomal vesicles

Gábor Bánhegyi, Lorenzo Lusini, Ferenc Puskás, Ranieri Rossi, Rosella Fulceri, Lásazló Braun, Valéria Mile, Paolo Di Simplicio, József Mandl, Angelo Benedetti

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

A bi-directional, saturable transport of glutathione (GSH) was found in rat liver microsomal vesicles. GSH transport could be inhibited by the anion transport blockers flufenamic acid and 4,4'-diisothiocyanostilbene-2,2'- disulfonic acid. A part of GSH taken up by the vesicles was metabolized to glutathione disulfide (GSSG) in the lumen. Microsomal membrane was virtually nonpermeable toward GSSG; accordingly, GSSG generated in the microsomal lumen could hardly exit. Therefore, GSH transport, contrary to previous assumptions, is preferred in the endoplasmic reticulum, and GSSG entrapped and accumulated in the lumen creates the oxidized state of its redox buffer.

Original languageEnglish
Pages (from-to)12213-12216
Number of pages4
JournalJournal of Biological Chemistry
Volume274
Issue number18
DOIs
Publication statusPublished - Apr 30 1999

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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