Preferential regeneration of the NADPH: Protochlorophyllide oxidoreductase oligomer complexes in pea epicotyls after bleaching

Andrea Szenzenstein, Annamária Kósa, K. Solymosi, E. Sárvári, B. Böddi

Research output: Contribution to journalArticle

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Abstract

The regeneration and stability of the NADPH:protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) enzyme complexes were studied in bleached epicotyls of 9-day-old dark-germinated pea (Pisum sativum L. cv. Zsuzsi) seedlings. Middle segments were illuminated with 1300 mol m-2 s -1photon flux density (PFD) white light and subsequently incubated in total darkness for 4-24 h at 24°C. Almost the full amount of protochlorophyllide (Pchlide) was degraded after 60 min illumination. The preferential regeneration of the 655 nm emitting Pchlide form was observed after 4 h dark incubation; the accumulation of the short-wavelength Pchlide form - dominating in epicotyls of dark-grown seedling - required 18-24 h dark. The Pchlide content of bleached samples was around 2.5% of that of the etiolated samples; after 4 h of dark incubation this value increased to 4-7%. Polyacrylamide gel electrophoresis and western blot showed that the amount of the POR protein decreased to about 50% during bleaching; after 4 h regeneration it reached almost the same level as that of dark-grown samples. We concluded that much more POR protein compared with Pchlide pigment remained stable during bleaching and the non-destroyed POR units were able to form preferentially oligomers during the dark-regeneration which could collect de novo synthesized Pchlide into 655 nm emitting complexes. These data indicate the high stability of the POR protein in pea epicotyls and the importance of the molecular environment in stimulating the aggregation of POR units.

Original languageEnglish
Pages (from-to)102-112
Number of pages11
JournalPhysiologia Plantarum
Volume138
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

protochlorophyllide reductase
Protochlorophyllide
protochlorophyllides
epicotyls
Peas
oxidoreductases
bleaching
NADP (coenzyme)
Regeneration
peas
Seedlings
Protein Stability
Darkness
pea protein
Lighting
seedlings
white light
Polyacrylamide Gel Electrophoresis
sampling
Proteins

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Physiology

Cite this

Preferential regeneration of the NADPH : Protochlorophyllide oxidoreductase oligomer complexes in pea epicotyls after bleaching. / Szenzenstein, Andrea; Kósa, Annamária; Solymosi, K.; Sárvári, E.; Böddi, B.

In: Physiologia Plantarum, Vol. 138, No. 1, 01.2010, p. 102-112.

Research output: Contribution to journalArticle

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