Predictive motifs derived from cytosine methyltransferases

János Pósfai, Ashok S. Bhagwat, G. Pósfai, Richard J. Roberts

Research output: Contribution to journalArticle

415 Citations (Scopus)

Abstract

Thirteen bacterial DNA methyltransferases that catalyze the formation of 5-methylcytosine within specific DNA sequences possess related structures. Similar building blocks (motifs), containing invariant positions, can be found in the same order in all thirteen sequences. Five of these blocks are highly conserved while a further five contain weaker similarities. One block, which has the most invariant residues, contains the proline-cysteine dipeptide of the proposed catalytic site. A region in the second half of each sequence is unusually variable both in length and sequence composition. Those methyltransferases that exhibit significant homology in this region share common specificity in DNA recognition. The five highly conserved motifs can be used to discriminate the known 5-methylcytosine forming methyltransferases from all other methyltransferases of known sequence, and from all other identified proteins in the PIR, GenBank and EMBL databases. These five motifs occur in a mammalian methyltransferase responsible for the formation of 5-methylcytosine within CG dinucleotides. By searching the unidentified open reading frames present in the GenBank and EMBL databases, two potential 5-methylcytosine forming methyltransferases have been found.

Original languageEnglish
Pages (from-to)2421-2435
Number of pages15
JournalNucleic Acids Research
Volume17
Issue number7
DOIs
Publication statusPublished - Apr 11 1989

Fingerprint

Cytosine
Methyltransferases
5-Methylcytosine
DNA
DNA sequences
Nucleic Acid Databases
Proteins
Open Reading Frames
Invariant
Chemical analysis
DNA Sequence
Databases
Building Blocks
Specificity
Homology
Bacterial DNA
Dipeptides
Protein
Proline
Cysteine

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Predictive motifs derived from cytosine methyltransferases. / Pósfai, János; Bhagwat, Ashok S.; Pósfai, G.; Roberts, Richard J.

In: Nucleic Acids Research, Vol. 17, No. 7, 11.04.1989, p. 2421-2435.

Research output: Contribution to journalArticle

Pósfai, János ; Bhagwat, Ashok S. ; Pósfai, G. ; Roberts, Richard J. / Predictive motifs derived from cytosine methyltransferases. In: Nucleic Acids Research. 1989 ; Vol. 17, No. 7. pp. 2421-2435.
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