Prediction of protein disorder at the domain level

Z. Dosztányi, Márk Sándor, Peter Tompa, I. Simon

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Intrinsically disordered/unstructured proteins exist in a highly flexible conformational state largely devoid of secondary structural elements and tertiary contacts. Despite their lack of a well defined structure, these proteins often fulfill essential regulatory functions. The intrinsic lack of structure confers functional advantages on these proteins, allowing them to adopt multiple conformations and to bind to different binding partners. The structural flexibility of disordered regions hampers efforts solving structures at high resolution by X-ray crystallography and/or NMR. Removing such proteins/regions from high-throughput structural genomics pipelines would be of significant benefit in terms of cost and success rate. In this paper we outline the theoretical background of structural disorder, and review bioinformatic predictors that can be used to delineate regions most likely to be amenable for structure determination. The primary focus of our review is the interpretation of prediction results in a way that enables segmentation of proteins to separate ordered domains from disordered regions.

Original languageEnglish
Pages (from-to)161-171
Number of pages11
JournalCurrent Protein and Peptide Science
Volume8
Issue number2
DOIs
Publication statusPublished - Apr 2007

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Intrinsically Disordered Proteins
Proteins
X ray crystallography
X Ray Crystallography
Bioinformatics
Genomics
Computational Biology
Conformations
Pipelines
Throughput
Nuclear magnetic resonance
Costs and Cost Analysis
Costs

Keywords

  • Detection of protein disorder
  • High-throughput structural studies
  • Intrinsically unstructured protein
  • Natively unfolded protein
  • Screening disordered protein
  • Structural genomics
  • Target prioritization

ASJC Scopus subject areas

  • Biochemistry

Cite this

Prediction of protein disorder at the domain level. / Dosztányi, Z.; Sándor, Márk; Tompa, Peter; Simon, I.

In: Current Protein and Peptide Science, Vol. 8, No. 2, 04.2007, p. 161-171.

Research output: Contribution to journalArticle

Dosztányi, Z. ; Sándor, Márk ; Tompa, Peter ; Simon, I. / Prediction of protein disorder at the domain level. In: Current Protein and Peptide Science. 2007 ; Vol. 8, No. 2. pp. 161-171.
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