Prediction of migration behavior of oligonucleotides in capillary gel electrophoresis

A. Guttman, Robert J. Nelson, Nelson Cooke

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The influence of the primary structure (base composition) on the electrophoretic migration properties of single-stranded oligodeoxyribonucleotides in capillary polyacrylamide gel electrophoresis was investigated using homo- and heterooligomers under denaturing and non-denaturing conditions. Homooligodeoxyribonucleotides of equal chain lenghts but of different base composition showed significant differences in mobility. In addition, the migration properties of heterooligomers were found to be highly dependent on their base composition. A simple equation is presented for predicting relative migration times using denaturing and non-denaturing polyacrylamide capillary gel electrophoresis. Orange-G was used as an internal standard and as the basis of the relative migration time calculations. Examples are presented using homo- and heterooligomers in the 10-20-mer range to show the correlation of the primary structure and their predicted and observed migration rates.

Original languageEnglish
Pages (from-to)297-303
Number of pages7
JournalJournal of Chromatography A
Volume593
Issue number1-2
DOIs
Publication statusPublished - Feb 28 1992

Fingerprint

Base Composition
Capillary Electrophoresis
Electrophoresis
Oligonucleotides
Gels
Chemical analysis
Native Polyacrylamide Gel Electrophoresis
Oligodeoxyribonucleotides
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Prediction of migration behavior of oligonucleotides in capillary gel electrophoresis. / Guttman, A.; Nelson, Robert J.; Cooke, Nelson.

In: Journal of Chromatography A, Vol. 593, No. 1-2, 28.02.1992, p. 297-303.

Research output: Contribution to journalArticle

Guttman, A. ; Nelson, Robert J. ; Cooke, Nelson. / Prediction of migration behavior of oligonucleotides in capillary gel electrophoresis. In: Journal of Chromatography A. 1992 ; Vol. 593, No. 1-2. pp. 297-303.
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