Precursor nucleotides at the 5' end are not required for processing by RNase E at the 3' end of 5-S rRNA.

J. Szeberényi, M. K. Roy, D. Apirion

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

7-S RNA, a single-site substrate for the processing enzyme RNase E of Escherichia coli, consists of p5 rRNA (the precursors of 5-S RNA) and the 3'-end region of the rRNA transcript which is mainly a termination stem and loop. The 7-S RNA studied here was derived mainly from the rRNA gene cluster rrnD, carried in a multicopy plasmid. It contains four different populations of molecules that differ from each other at their 5' ends only: the shortest species has the 5' end of the mature 5-S rRNA, while the others are one, two and three nucleotides longer. The four different 5'-end forms were separated in a long sequencing gel. Processing of these forms and unfractionated 7-S RNA by RNase E in vitro, showed that all forms, even the shortest one, can be processed to p5 rRNA. Since the extra nucleotides at the 5' end of the molecule could be base-paired with the region of 7-S RNA where RNase E cuts, it is concluded that this double-stranded structure is not required for the action of RNase E in separating the 3' end of p5 rRNA from the termination stem.

Original languageEnglish
Pages (from-to)321-326
Number of pages6
JournalEuropean Journal of Biochemistry
Volume136
Issue number2
Publication statusPublished - Nov 2 1983

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Nucleotides
RNA
Processing
Escherichia coli
Molecules
RNA Precursors
Multigene Family
rRNA Genes
Plasmids
Genes
Gels
ribonuclease E
Substrates
Enzymes
Population

ASJC Scopus subject areas

  • Biochemistry

Cite this

Precursor nucleotides at the 5' end are not required for processing by RNase E at the 3' end of 5-S rRNA. / Szeberényi, J.; Roy, M. K.; Apirion, D.

In: European Journal of Biochemistry, Vol. 136, No. 2, 02.11.1983, p. 321-326.

Research output: Contribution to journalArticle

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