Preconditioning with volatile anaesthetic sevoflurane in ischemic retinal lesion in rats

K. Szabadfi, Bese Danyadi, P. Kiss, Sridharan Manavalan, R. Gábriel, D. Reglodi, A. Tamás, Domonkos Trasy, Istvan Batai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Volatile anaesthetic agents have been recognized for their neuroprotective properties since the 1960s. However, little is known regarding the potential retinoprotective effects of preconditioning by anaesthetic drugs. Retinal ischemia can be modeled by permanent bilateral common carotid artery occlusion (BCCAO). Here we studied the degree of ischemic injury with preconditioning by sevoflurane in the rat retina. During the BCCAO operation and preconditioning Wistar rats were anaesthetized with 1 MAC of sevoflurane. The oxygen, carbon dioxide, and anaesthetic vapor concentration in the anaesthetizing box was monitored with a gas analyzer. We examined 4 groups: non-and preconditioning groups in control and BCCAO animals. The duration of preconditioning period was 1 h and it was performed 1 day before BCCAO. The retinas were processed for histological evaluation after 2 weeks survival to determine the cell number in the ganglion cell layer and the thickness of the whole retina and that of all retinal layers. BCCAO-induced retinal ischemic injury was ameliorated by sevoflurane preconditioning. Retinal thickness and the cell number in the ganglion cell layer were more retained in preconditioned animals after BCCAO compared to non-preconditioned group. These results suggest that preconditioning using sevoflurane could provide a new perspective in retinoprotective strategies.

Original languageEnglish
Pages (from-to)565-569
Number of pages5
JournalJournal of Molecular Histology
Volume43
Issue number5
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Common Carotid Artery
Anesthetics
Retina
Ganglia
Cell Count
Wounds and Injuries
Carbon Dioxide
sevoflurane
Wistar Rats
Ischemia
Gases
Oxygen
Control Groups

Keywords

  • Ischemia
  • Retina
  • Retinoprotection
  • Sevoflurane preconditioning

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Physiology

Cite this

Preconditioning with volatile anaesthetic sevoflurane in ischemic retinal lesion in rats. / Szabadfi, K.; Danyadi, Bese; Kiss, P.; Manavalan, Sridharan; Gábriel, R.; Reglodi, D.; Tamás, A.; Trasy, Domonkos; Batai, Istvan.

In: Journal of Molecular Histology, Vol. 43, No. 5, 10.2012, p. 565-569.

Research output: Contribution to journalArticle

@article{85cf52b8b5b84faa8dde30bea47a8e41,
title = "Preconditioning with volatile anaesthetic sevoflurane in ischemic retinal lesion in rats",
abstract = "Volatile anaesthetic agents have been recognized for their neuroprotective properties since the 1960s. However, little is known regarding the potential retinoprotective effects of preconditioning by anaesthetic drugs. Retinal ischemia can be modeled by permanent bilateral common carotid artery occlusion (BCCAO). Here we studied the degree of ischemic injury with preconditioning by sevoflurane in the rat retina. During the BCCAO operation and preconditioning Wistar rats were anaesthetized with 1 MAC of sevoflurane. The oxygen, carbon dioxide, and anaesthetic vapor concentration in the anaesthetizing box was monitored with a gas analyzer. We examined 4 groups: non-and preconditioning groups in control and BCCAO animals. The duration of preconditioning period was 1 h and it was performed 1 day before BCCAO. The retinas were processed for histological evaluation after 2 weeks survival to determine the cell number in the ganglion cell layer and the thickness of the whole retina and that of all retinal layers. BCCAO-induced retinal ischemic injury was ameliorated by sevoflurane preconditioning. Retinal thickness and the cell number in the ganglion cell layer were more retained in preconditioned animals after BCCAO compared to non-preconditioned group. These results suggest that preconditioning using sevoflurane could provide a new perspective in retinoprotective strategies.",
keywords = "Ischemia, Retina, Retinoprotection, Sevoflurane preconditioning",
author = "K. Szabadfi and Bese Danyadi and P. Kiss and Sridharan Manavalan and R. G{\'a}briel and D. Reglodi and A. Tam{\'a}s and Domonkos Trasy and Istvan Batai",
year = "2012",
month = "10",
doi = "10.1007/s10735-012-9426-y",
language = "English",
volume = "43",
pages = "565--569",
journal = "Journal of Molecular Histology",
issn = "1567-2379",
publisher = "Springer Netherlands",
number = "5",

}

TY - JOUR

T1 - Preconditioning with volatile anaesthetic sevoflurane in ischemic retinal lesion in rats

AU - Szabadfi, K.

AU - Danyadi, Bese

AU - Kiss, P.

AU - Manavalan, Sridharan

AU - Gábriel, R.

AU - Reglodi, D.

AU - Tamás, A.

AU - Trasy, Domonkos

AU - Batai, Istvan

PY - 2012/10

Y1 - 2012/10

N2 - Volatile anaesthetic agents have been recognized for their neuroprotective properties since the 1960s. However, little is known regarding the potential retinoprotective effects of preconditioning by anaesthetic drugs. Retinal ischemia can be modeled by permanent bilateral common carotid artery occlusion (BCCAO). Here we studied the degree of ischemic injury with preconditioning by sevoflurane in the rat retina. During the BCCAO operation and preconditioning Wistar rats were anaesthetized with 1 MAC of sevoflurane. The oxygen, carbon dioxide, and anaesthetic vapor concentration in the anaesthetizing box was monitored with a gas analyzer. We examined 4 groups: non-and preconditioning groups in control and BCCAO animals. The duration of preconditioning period was 1 h and it was performed 1 day before BCCAO. The retinas were processed for histological evaluation after 2 weeks survival to determine the cell number in the ganglion cell layer and the thickness of the whole retina and that of all retinal layers. BCCAO-induced retinal ischemic injury was ameliorated by sevoflurane preconditioning. Retinal thickness and the cell number in the ganglion cell layer were more retained in preconditioned animals after BCCAO compared to non-preconditioned group. These results suggest that preconditioning using sevoflurane could provide a new perspective in retinoprotective strategies.

AB - Volatile anaesthetic agents have been recognized for their neuroprotective properties since the 1960s. However, little is known regarding the potential retinoprotective effects of preconditioning by anaesthetic drugs. Retinal ischemia can be modeled by permanent bilateral common carotid artery occlusion (BCCAO). Here we studied the degree of ischemic injury with preconditioning by sevoflurane in the rat retina. During the BCCAO operation and preconditioning Wistar rats were anaesthetized with 1 MAC of sevoflurane. The oxygen, carbon dioxide, and anaesthetic vapor concentration in the anaesthetizing box was monitored with a gas analyzer. We examined 4 groups: non-and preconditioning groups in control and BCCAO animals. The duration of preconditioning period was 1 h and it was performed 1 day before BCCAO. The retinas were processed for histological evaluation after 2 weeks survival to determine the cell number in the ganglion cell layer and the thickness of the whole retina and that of all retinal layers. BCCAO-induced retinal ischemic injury was ameliorated by sevoflurane preconditioning. Retinal thickness and the cell number in the ganglion cell layer were more retained in preconditioned animals after BCCAO compared to non-preconditioned group. These results suggest that preconditioning using sevoflurane could provide a new perspective in retinoprotective strategies.

KW - Ischemia

KW - Retina

KW - Retinoprotection

KW - Sevoflurane preconditioning

UR - http://www.scopus.com/inward/record.url?scp=84871262210&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84871262210&partnerID=8YFLogxK

U2 - 10.1007/s10735-012-9426-y

DO - 10.1007/s10735-012-9426-y

M3 - Article

C2 - 22684245

AN - SCOPUS:84871262210

VL - 43

SP - 565

EP - 569

JO - Journal of Molecular Histology

JF - Journal of Molecular Histology

SN - 1567-2379

IS - 5

ER -