Preconditioning is a method that may reduce the negative side-effect of pneumoperitoneum

Sz Jávor, K. Shanava, E. Hocsák, M. Kürthy, J. Lantos, B. Borsiczky, I. Takács, Sz Horváth, B. Balatonyi, S. Ferencz, A. Ferencz, E. Rőth, G. Wéber

Research output: Contribution to journalArticle

Abstract

Increased intra-abdominal pressure during laparoscopy leads to hypoxia due to reduced blood flow. Aim of our study was to investigate whether preconditioning can reduce this negative effect of the pneumoperitoneum. Fifty female Wistar rats were used, divided into 5 groups. I: Sham operation (Sham), II: conventional pneumoperitoneum (PP), III: transvaginal pneumoperitoneum (TV), IV: preconditioning for 2.5 minutes in two cycles (Pre 2.5), V: preconditioning for 5 minutes (Pre 5). Malondialdehyde (MDA), reduced glutathione (GSH), sulfhydrylgroup (SH-) concentrations, superoxide-dismutase (SOD) and mieloperoxidase (MPO) activity, and anti-apoptotic pathway marker p-AKT level and inflammatory cytokine TNF-α were measured. SOD activity and GSH concentration were decreased in PP and TV groups comparing to Sham and preconditioning groups. MPO activity was decreased also in PP and TV groups comparing to the Sham group but in the preconditioning groups it has remained high. MDA concentration in plasma was increased in PP and TV groups comparing to Sham and preconditioning groups. There was no difference in the case of blood MDA and SH-concentrations between groups. Anti-apoptotic pathway marker p-AKT level was decreased in the TV group comparing to the sham and preconditioning groups. TNF-α level was increased in TV and preconditioning groups compared to the sham group. According to the results preconditioning can reduce negative effects of pneumoperitoneum.

Original languageEnglish
Pages (from-to)115-120
Number of pages6
JournalInterventional Medicine and Applied Science
Volume2
Issue number3
DOIs
Publication statusPublished - Sep 1 2010

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Pneumoperitoneum
Malondialdehyde
Superoxide Dismutase
Laparoscopy
Glutathione
Wistar Rats

Keywords

  • ischemia
  • laparoscopic surgery
  • oxidative stress
  • pneumoperitoneum
  • preconditioning

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Preconditioning is a method that may reduce the negative side-effect of pneumoperitoneum. / Jávor, Sz; Shanava, K.; Hocsák, E.; Kürthy, M.; Lantos, J.; Borsiczky, B.; Takács, I.; Horváth, Sz; Balatonyi, B.; Ferencz, S.; Ferencz, A.; Rőth, E.; Wéber, G.

In: Interventional Medicine and Applied Science, Vol. 2, No. 3, 01.09.2010, p. 115-120.

Research output: Contribution to journalArticle

Jávor, Sz ; Shanava, K. ; Hocsák, E. ; Kürthy, M. ; Lantos, J. ; Borsiczky, B. ; Takács, I. ; Horváth, Sz ; Balatonyi, B. ; Ferencz, S. ; Ferencz, A. ; Rőth, E. ; Wéber, G. / Preconditioning is a method that may reduce the negative side-effect of pneumoperitoneum. In: Interventional Medicine and Applied Science. 2010 ; Vol. 2, No. 3. pp. 115-120.
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AB - Increased intra-abdominal pressure during laparoscopy leads to hypoxia due to reduced blood flow. Aim of our study was to investigate whether preconditioning can reduce this negative effect of the pneumoperitoneum. Fifty female Wistar rats were used, divided into 5 groups. I: Sham operation (Sham), II: conventional pneumoperitoneum (PP), III: transvaginal pneumoperitoneum (TV), IV: preconditioning for 2.5 minutes in two cycles (Pre 2.5), V: preconditioning for 5 minutes (Pre 5). Malondialdehyde (MDA), reduced glutathione (GSH), sulfhydrylgroup (SH-) concentrations, superoxide-dismutase (SOD) and mieloperoxidase (MPO) activity, and anti-apoptotic pathway marker p-AKT level and inflammatory cytokine TNF-α were measured. SOD activity and GSH concentration were decreased in PP and TV groups comparing to Sham and preconditioning groups. MPO activity was decreased also in PP and TV groups comparing to the Sham group but in the preconditioning groups it has remained high. MDA concentration in plasma was increased in PP and TV groups comparing to Sham and preconditioning groups. There was no difference in the case of blood MDA and SH-concentrations between groups. Anti-apoptotic pathway marker p-AKT level was decreased in the TV group comparing to the sham and preconditioning groups. TNF-α level was increased in TV and preconditioning groups compared to the sham group. According to the results preconditioning can reduce negative effects of pneumoperitoneum.

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