Preconditioning decreases ischemia/reperfusion-induced peroxynitrite formation

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45 Citations (Scopus)

Abstract

The role for peroxynitrite (ONOO-) in the mechanism of preconditioning is not known. Therefore, we studied effects of preconditioning and subsequent ischemia/reperfusion on myocardial ONOO- formation in isolated rat hearts. Hearts were subjected to a preconditioning protocol (three intermittent periods of global ischemia/reperfusion of 5 min duration each) followed by a test ischemia/reperfusion (30 min global ischemia and 15 min reperfusion). When compared to nonpreconditioned controls, preceding preconditioning improved postischemic cardiac performance and significantly decreased test ischemia/reperfusion-induced formation of free nitrotyrosine measured in the perfusate as a marker for cardiac endogenous ONOO- formation. During preconditioning, however, the first period of ischemia/reperfusion increased nitrotyrosine formation, which was attenuated after the third period of ischemia/reperfusion. We conclude that classic preconditioning inhibits ischemia/reperfusion-induced cardiac formation of ONOO- and that subsequent periods of ischemia/reperfusion result in a gradual attenuation of ischemia/reperfusion-induced ONOO- generation. This mechanism might be involved in ischemic adaptation of the heart.

Original languageEnglish
Pages (from-to)1217-1219
Number of pages3
JournalBiochemical and biophysical research communications
Volume285
Issue number5
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Ischemia/reperfusion
  • Nitrotyrosine
  • Peroxynitrite
  • Preconditioning
  • Rat heart

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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