Pre-treatment and post-treatment with α-tocopherol attenuates hippocampal neuronal damage in experimental cerebral hypoperfusion

Anita Annaházi, Éva Mracskó, Zoltán Süle, E. Karg, B. Penke, F. Bari, E. Farkas

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

α-Tocopherol, a potent antioxidant, has been widely investigated as a dietary supplement with which to reduce the risk of atherosclerosis, and has recently been considered as a potential supplement to moderate oxidative neuronal damage in Alzheimer's disease patients. Since α-tocopherol appears beneficial in vascular and neurodegenerative disorders, we set out to identify its neuroprotective action in a rat model of chronic cerebral hypoperfusion-induced brain injury. The bilateral common carotid arteries of male Wistar rats were permanently occluded (2VO). Sham-operated animals served as controls. Half of the animals were pre- or post-treated repeatedly with α-tocopherol (5 × 100 mg/kg daily, i.p.), the other half receiving only soybean oil, the α-tocopherol vehicle. One week after the onset of 2VO, the spatial learning capacity of the animals was assessed in the Morris water maze. After testing, hippocampal slices were stained with cresyl violet in order to examine the pyramidal cell layer integrity. The density of microtubule-associated protein-2 (MAP-2)-positive dendrites and the OX-42-labeled microglial activation level were determined immunocytochemically. Finally, α-tocopherol was determined in the peripheral tissues, blood and brain. α-Tocopherol moderated the 2VO-induced learning impairment. The various forms of α-tocopherol treatment, and particularly the post-treatment, prevented the 2VO-induced pyramidal cell death and the activation of microglia in the hippocampus CA1 region, and the degeneration of MAP-2-positive dendrites in the CA3 region. The α-tocopherol concentration was elevated in the peripheral tissues and the blood, but not in the brain. The data indicate that α-tocopherol, particularly when administered as post-treatment, is neuroprotective in chronic cerebral hypoperfusion.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalEuropean Journal of Pharmacology
Volume571
Issue number2-3
DOIs
Publication statusPublished - Oct 1 2007

Fingerprint

Tocopherols
Microtubule-Associated Proteins
Therapeutics
Pyramidal Cells
Dendrites
Soybean Oil
Common Carotid Artery
Brain
Microglia
Dietary Supplements
Neurodegenerative Diseases
Brain Injuries
Blood Vessels
Wistar Rats
Hippocampus
Atherosclerosis
Alzheimer Disease
Cell Death
Antioxidants
Learning

Keywords

  • α-Tocopherol
  • Carotid occlusion
  • Hippocampus
  • Learning
  • Neurodegeneration

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Pre-treatment and post-treatment with α-tocopherol attenuates hippocampal neuronal damage in experimental cerebral hypoperfusion. / Annaházi, Anita; Mracskó, Éva; Süle, Zoltán; Karg, E.; Penke, B.; Bari, F.; Farkas, E.

In: European Journal of Pharmacology, Vol. 571, No. 2-3, 01.10.2007, p. 120-128.

Research output: Contribution to journalArticle

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