Practical sample pretreatment techniques coupled with capillary electrophoresis for real samples in complex matrices

Gabor Jarvas, Andras Guttman, Natalia Miękus, Tomasz Bączek, Sunkyung Jeong, Doo Soo Chung, Vladimir Pätoprstý, Marián Masár, Milan Hutta, Vladimira Datinská, Frantisek Foret

Research output: Contribution to journalReview article

Abstract

By coupling a sample pretreatment technique of sample clean up and enrichment power with capillary electrophoresis (CE) of high-performance separation, the task of analyzing trace analytes in a complex matrix such as a biological sample can be carried out successfully with ease. This review aims for providing an overview of strategies to couple sample pretreatment techniques with capillary and related microscale (e.g., microchip) electrophoresis, practically adoptable in an automatic manner, without requiring serious modification of existing instruments to install sophisticated interfaces. In-line sample pretreatment techniques based on liquid phase microextraction performed before sample injection and on-line sample preconcentration techniques performed during or after sample injection are discussed with emphasis on the applicability to samples of high conductivity, commonly encountered for biological samples. An overview of the recent developments in microfluidic immobilized enzymatic microreactors which fit excellently to microchip CE is also given.

Original languageEnglish
Article number115702
JournalTrAC - Trends in Analytical Chemistry
Volume122
DOIs
Publication statusPublished - Jan 2020

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Capillary electrophoresis
Chemical reactors
Phase separation
Microprocessor chips
Electrophoresis
Microfluidics
Liquids

Keywords

  • Analyte preconcentration
  • Capillary electrophoresis
  • Liquid phase separation
  • Microreactors
  • Sample pretreatment

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Practical sample pretreatment techniques coupled with capillary electrophoresis for real samples in complex matrices. / Jarvas, Gabor; Guttman, Andras; Miękus, Natalia; Bączek, Tomasz; Jeong, Sunkyung; Chung, Doo Soo; Pätoprstý, Vladimir; Masár, Marián; Hutta, Milan; Datinská, Vladimira; Foret, Frantisek.

In: TrAC - Trends in Analytical Chemistry, Vol. 122, 115702, 01.2020.

Research output: Contribution to journalReview article

Jarvas, G, Guttman, A, Miękus, N, Bączek, T, Jeong, S, Chung, DS, Pätoprstý, V, Masár, M, Hutta, M, Datinská, V & Foret, F 2020, 'Practical sample pretreatment techniques coupled with capillary electrophoresis for real samples in complex matrices', TrAC - Trends in Analytical Chemistry, vol. 122, 115702. https://doi.org/10.1016/j.trac.2019.115702
Jarvas, Gabor ; Guttman, Andras ; Miękus, Natalia ; Bączek, Tomasz ; Jeong, Sunkyung ; Chung, Doo Soo ; Pätoprstý, Vladimir ; Masár, Marián ; Hutta, Milan ; Datinská, Vladimira ; Foret, Frantisek. / Practical sample pretreatment techniques coupled with capillary electrophoresis for real samples in complex matrices. In: TrAC - Trends in Analytical Chemistry. 2020 ; Vol. 122.
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AU - Chung, Doo Soo

AU - Pätoprstý, Vladimir

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