PR-1 protein inhibits the differentiation of rust infection hyphae in leaves of acquired resistant broad bean

Martina Rauscher, A. Ádám, Sabine Wirtz, Richard Guggenheim, Kurt Mendgen, Holger B. Deising

Research output: Contribution to journalArticle

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Abstract

Treatment of broad bean leaves with salicylic acid (SA) or 2,6-dichloro-isonicotinic acid (DCINA) induces resistance against the rust fungus Uromyces fabae resulting in reduced rust pustule density. Light-microscopy studies showed that in induced resistant plants the rust fungus is inhibited immediately after penetration through the stomatal pore. The differentiation of infection structures growing within the intercellular space of the leaf, i.e. infection hyphae and haustorial mother cells, is inhibited. Furthermore, low-temperature scanning electron microscopy studies of freeze fractures revealed protrusions at the tips of infection hyphae growing in induced resistant broad bean leaves. Treatment of in vitro-differentiating rust infection structures with intercellular fluids (IFs) from induced resistant plants confirmed that the fungus is sensitive towards an apoplastic anti-fungal activity only after having formed appressoria. Other legume rusts such as U. vignae and U. appendiculatus were likewise inhibited in the presence of IF from SA-treated broad bean leaves. Heterologous antibodies were used to study changes in the extracellular pathogenesis-related (PR) protein pattern after resistance induction. Western blots indicated that chitinases and β-1,3-glucanases were present in both induced and control plants. In contrast, PR-1 proteins were newly synthesized in response to SA or DCINA application. The major induced PR-1 protein was purified and exhibited strong differentiation-inhibiting activity towards U. fabae infection structures. We conclude that the inhibition of rust infection hyphae in acquired resistant broad bean plants is mainly due to the anti-fungal activity of this induced basic PR-1 protein.

Original languageEnglish
Pages (from-to)625-633
Number of pages9
JournalPlant Journal
Volume19
Issue number6
DOIs
Publication statusPublished - Sep 1999

Fingerprint

rust diseases
Hyphae
faba beans
hyphae
pathogenesis
Uromyces viciae-fabae
salicylic acid
extracellular fluids
Salicylic Acid
Isonicotinic Acids
Infection
infection
fungi
leaves
Proteins
Fungi
proteins
Extracellular Fluid
acid tolerance
appressoria

ASJC Scopus subject areas

  • Plant Science

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PR-1 protein inhibits the differentiation of rust infection hyphae in leaves of acquired resistant broad bean. / Rauscher, Martina; Ádám, A.; Wirtz, Sabine; Guggenheim, Richard; Mendgen, Kurt; Deising, Holger B.

In: Plant Journal, Vol. 19, No. 6, 09.1999, p. 625-633.

Research output: Contribution to journalArticle

Rauscher, Martina ; Ádám, A. ; Wirtz, Sabine ; Guggenheim, Richard ; Mendgen, Kurt ; Deising, Holger B. / PR-1 protein inhibits the differentiation of rust infection hyphae in leaves of acquired resistant broad bean. In: Plant Journal. 1999 ; Vol. 19, No. 6. pp. 625-633.
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