PP2A regulates autophagy in two alternative ways in Drosophila

Ágnes Bánréti, Tamás Lukácsovich, György Csikós, M. Erdélyi, M. Sass

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Protein phosphatase 2A (PP2A) holoenzyme is a heterotrimeric complex, consisting of A, B and C subunits. The catalytic subunit PP2A-C (microtubule star/mts) binds to the C-terminal part of the scaffold protein PP2A-A (PP2A-29B). In Drosophila, there are three different forms of B subunits (widerborst/wdb, twins/tws and PP2A-B′), which determine the subcellular localization and substrate specificity of the holoenzyme. Previous studies demonstrated that PP2A is involved in the control of TOR-dependent autophagy both in yeast and mammals. Furthermore, in Drosophila, wdb genetically interacts with the PtdIns3K/PTEN/Akt signaling cascade, which is a main upstream regulatory system of dTOR. Here we demonstrate that in Drosophila, two different PP2A complexes (containing B' or wdb subunit) play essential roles in the regulation of starvation-induced autophagy. The PP2A-A/wdb/C complex acts upstream of dTOR, whereas the PP2A-A/B′/ C complex functions as a target of dTOR and may regulate the elongation of autophagosomes and their subsequent fusion with lysosomes. We also identified three Drosophila Atg orthologs (Atg14, Atg17 and Atg101), which represent potential targets of the PP2A-A/B′/C complex during autophagy.

Original languageEnglish
Pages (from-to)623-636
Number of pages14
JournalAutophagy
Volume8
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Protein Phosphatase 2
Autophagy
Drosophila
Holoenzymes
Phosphoprotein Phosphatases
Substrate Specificity
Starvation
Lysosomes
Microtubules
Mammals
Catalytic Domain
Yeasts

Keywords

  • Autophagy
  • Drosophila
  • Larval fat body
  • mts
  • PP2A
  • srp
  • TOR
  • wdb

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

PP2A regulates autophagy in two alternative ways in Drosophila. / Bánréti, Ágnes; Lukácsovich, Tamás; Csikós, György; Erdélyi, M.; Sass, M.

In: Autophagy, Vol. 8, No. 4, 04.2012, p. 623-636.

Research output: Contribution to journalArticle

Bánréti, Ágnes ; Lukácsovich, Tamás ; Csikós, György ; Erdélyi, M. ; Sass, M. / PP2A regulates autophagy in two alternative ways in Drosophila. In: Autophagy. 2012 ; Vol. 8, No. 4. pp. 623-636.
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