Powdery mildew induces defense-oriented reprogramming of the transcriptome in a susceptible but not in a resistant grapevine

Raymond W M Fung, Martin Gonzalo, C. Fekete, Laszlo G. Kovacs, Yan He, Ellen Marsh, Lauren M. McIntyre, Daniel P. Schachtman, Wenping Qiu

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Grapevines exhibit a wide spectrum of resistance to the powdery mildew fungus (PM), Erysiphe necator (Schw.) Burr., but little is known about the transcriptional basis of the defense to PM. Our microscopic observations showed that PM produced less hyphal growth and induced more brown-colored epidermal cells on leaves of PM-resistant Vitis aestivalis 'Norton' than on leaves of PM-susceptible Vitis vinifera 'Cabernet sauvignon'. We found that endogenous salicylic acid levels were higher in V. aestivalis than in V. vinifera in the absence of the fungus and that salicylic acid levels increased in V. vinifera at 120 h postinoculation with PM. To test the hypothesis that gene expression differences would be apparent when V. aestivalis and V. vinifera were mounting a response to PM, we conducted a comprehensive Vitis GeneChip analysis. We examined the transcriptome at 0, 4, 8, 12, 24, and 48 h postinoculation with PM. We found only three PM-responsive transcripts in V. aestivalis and 625 in V. vinifera. There was a significant increase in the abundance of transcripts encoding ENHANCED DISEASE SUSCEPTIBILITY1, mitogen-activated protein kinase kinase, WRKY, PATHOGENESIS-RELATED1, PATHOGENESIS-RELATED10, and stilbene synthase in PM-infected V. vinifera, suggesting an induction of the basal defense response. The overall changes in the PM-responsive V. vinifera transcriptome also indicated a possible reprogramming of metabolism toward the increased synthesis of the secondary metabolites. These results suggested that resistance to PM in V. aestivalis was not associated with overall reprogramming of the transcriptome. However, PM induced defense-oriented transcriptional changes in V. vinifera.

Original languageEnglish
Pages (from-to)236-249
Number of pages14
JournalPlant Physiology
Volume146
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

powdery mildew
Transcriptome
transcriptome
Fungi
Vitis
fungi
Vitis aestivalis
Vitis vinifera
Salicylic Acid
salicylic acid
Necator
pathogenesis
Uncinula necator
mitogen-activated protein kinase kinase
Mitogen-Activated Protein Kinase Kinases
secondary metabolites
leaves

ASJC Scopus subject areas

  • Plant Science

Cite this

Powdery mildew induces defense-oriented reprogramming of the transcriptome in a susceptible but not in a resistant grapevine. / Fung, Raymond W M; Gonzalo, Martin; Fekete, C.; Kovacs, Laszlo G.; He, Yan; Marsh, Ellen; McIntyre, Lauren M.; Schachtman, Daniel P.; Qiu, Wenping.

In: Plant Physiology, Vol. 146, No. 1, 01.2008, p. 236-249.

Research output: Contribution to journalArticle

Fung, RWM, Gonzalo, M, Fekete, C, Kovacs, LG, He, Y, Marsh, E, McIntyre, LM, Schachtman, DP & Qiu, W 2008, 'Powdery mildew induces defense-oriented reprogramming of the transcriptome in a susceptible but not in a resistant grapevine', Plant Physiology, vol. 146, no. 1, pp. 236-249. https://doi.org/10.1104/pp.107.108712
Fung, Raymond W M ; Gonzalo, Martin ; Fekete, C. ; Kovacs, Laszlo G. ; He, Yan ; Marsh, Ellen ; McIntyre, Lauren M. ; Schachtman, Daniel P. ; Qiu, Wenping. / Powdery mildew induces defense-oriented reprogramming of the transcriptome in a susceptible but not in a resistant grapevine. In: Plant Physiology. 2008 ; Vol. 146, No. 1. pp. 236-249.
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