Powdery mildew-induced hormonal and photosynthetic changes in barley near isogenic lines carrying various resistant genes

Diana Saja, Anna Janeczko, Balázs Barna, Andrzej Skoczowski, Michał Dziurka, Andrzej Kornaś, Gábor Gullner

Research output: Contribution to journalArticle

Abstract

The present work focused on the characterization of some physiological mechanisms activated upon powdery mildew inoculation of the susceptible barley cultivar Ingrid and its near-isogenic lines (NILs) carrying various resistant genes (Mla, Mlg and mlo). After inoculation with Blumeria graminis f. sp. hordei (Bgh), measurements of leaf reflectance and chlorophyll a fluorescence were performed 3 and 7 day post-inoculation (dpi), while hormone assays were made 7 dpi. Bgh-inoculated resistant genotypes were characterized by lowered leaf reflectance parameters that correlated with carotenoids (CRI) and water content (WBI) in comparison to inoculated Ingrid. The PSII activity (i.e., Fv/Fm, ETo/CSm and P.I.ABS) strongly decreased in susceptible Ingrid leaves when the disease symptoms became visible 7 dpi. In Mla plants with visible hypersensitive spots the PSII activity decreased to a lesser extent. Inoculation resulted in a very slight decrease of photosynthesis at later stage of infection in Mlg plants, whereas in resistant mlo plants the PSII activity did not change. Chlorophyll a fluorescence measurements allowed presymptomatic detection of infection in Ingrid and Mla. Changes in the homeostasis of 22 phytohormones (cytokinins, auxins, gibberellins and the stress hormones JA, SA and ABA) in powdery mildew inoculated barley are discussed in relation to resistance against this biotrophic pathogen.

Original languageEnglish
Article number4536
Pages (from-to)1-23
Number of pages23
JournalInternational journal of molecular sciences
Volume21
Issue number12
DOIs
Publication statusPublished - Jun 2020

Keywords

  • Barley
  • Blumeria graminis
  • Chlorophyll a fluorescence
  • Leaf reflectance
  • Phytohormones
  • Resistance genes

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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