Nukleinsav fragmensek és rokon ligandumok foszfátesoportjai és a (CH3)2Sn(IV)2+ kölcsönhatásának igazolása potenciometriás és spektroszkópiás módszerekkel

Translated title of the contribution: Potentiometric and spectroscopic evidence for coordination of dimethyltin(IV)2+ to phosphate groups of DNA fragments and related ligands

A. Jancsó, László Nagy, Einar Sletten, Erlend Moldrheim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The co-ordination of dimethyltin(IV)2+ to nucleic acid fragments (5′-GMP, 5′-ATP and 5′-d(CGCGCG)2) and their sugar constituents (D-ribose and 2-deoxy-D-ribose) as ligands was investigated in aqueous solution by means of potentiorhetric titration and 1H and 31P NMR spectroscopic methods. The results showed that in acidic medium the phosphate groups can provide suitable sites for metal ion co-ordination, while the hydroxyl groups of the studied sugars or the sugar moieties of the two nucleotides play a role in this process in the higher pH range. The base moieties of 5′-GMP and 5′-ATP were not co-ordinated to dimethyltin(IV)2+. The stability constants of the complexes formed in the above systems were determined by pH-metric titration. The data revealed a stronger co-ordination ability of the triphosphate as compared with that of the monophosphate. The comparison of the stability constants of the D-ribose and 2-deoxy-D-ribose complexes showed that more stable species were formed when neighbouring alcoholic hydroxy groups were available for the co-ordination of metal ions. The observed chemical shift changes of the 31P NMR resonances, as compared with those measured for the metal-free systems, demonstrated that the phosphate groups of the DNA fragment [5′-d(CGCGCG)2] chains act as binding sites for dimethyltin(IV)2+ between pH 4.5 and 7. The 1D and 2D 1H NMR spectra indicated that the base and sugar moieties do not participate in the coordination process under these conditions.

Original languageHungarian
Pages (from-to)414-426
Number of pages13
JournalMagyar Kemiai Folyoirat, Kemiai Kozlemenyek
Volume106
Issue number10
Publication statusPublished - 2000

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Ribose
Sugars
Phosphates
Ligands
Guanosine Monophosphate
DNA
Nuclear magnetic resonance
Titration
Metal ions
Adenosine Triphosphate
Chemical shift
Hydroxyl Radical
Nucleic Acids
Nucleotides
Metals
Binding Sites
dimethyltin
tri(deoxycytidylic acid-deoxyguanylic acid)
tri(cytidylic acid-guanidylic acid)

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Nukleinsav fragmensek és rokon ligandumok foszfátesoportjai és a (CH3)2Sn(IV)2+ kölcsönhatásának igazolása potenciometriás és spektroszkópiás módszerekkel. / Jancsó, A.; Nagy, László; Sletten, Einar; Moldrheim, Erlend.

In: Magyar Kemiai Folyoirat, Kemiai Kozlemenyek, Vol. 106, No. 10, 2000, p. 414-426.

Research output: Contribution to journalArticle

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abstract = "The co-ordination of dimethyltin(IV)2+ to nucleic acid fragments (5′-GMP, 5′-ATP and 5′-d(CGCGCG)2) and their sugar constituents (D-ribose and 2-deoxy-D-ribose) as ligands was investigated in aqueous solution by means of potentiorhetric titration and 1H and 31P NMR spectroscopic methods. The results showed that in acidic medium the phosphate groups can provide suitable sites for metal ion co-ordination, while the hydroxyl groups of the studied sugars or the sugar moieties of the two nucleotides play a role in this process in the higher pH range. The base moieties of 5′-GMP and 5′-ATP were not co-ordinated to dimethyltin(IV)2+. The stability constants of the complexes formed in the above systems were determined by pH-metric titration. The data revealed a stronger co-ordination ability of the triphosphate as compared with that of the monophosphate. The comparison of the stability constants of the D-ribose and 2-deoxy-D-ribose complexes showed that more stable species were formed when neighbouring alcoholic hydroxy groups were available for the co-ordination of metal ions. The observed chemical shift changes of the 31P NMR resonances, as compared with those measured for the metal-free systems, demonstrated that the phosphate groups of the DNA fragment [5′-d(CGCGCG)2] chains act as binding sites for dimethyltin(IV)2+ between pH 4.5 and 7. The 1D and 2D 1H NMR spectra indicated that the base and sugar moieties do not participate in the coordination process under these conditions.",
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AB - The co-ordination of dimethyltin(IV)2+ to nucleic acid fragments (5′-GMP, 5′-ATP and 5′-d(CGCGCG)2) and their sugar constituents (D-ribose and 2-deoxy-D-ribose) as ligands was investigated in aqueous solution by means of potentiorhetric titration and 1H and 31P NMR spectroscopic methods. The results showed that in acidic medium the phosphate groups can provide suitable sites for metal ion co-ordination, while the hydroxyl groups of the studied sugars or the sugar moieties of the two nucleotides play a role in this process in the higher pH range. The base moieties of 5′-GMP and 5′-ATP were not co-ordinated to dimethyltin(IV)2+. The stability constants of the complexes formed in the above systems were determined by pH-metric titration. The data revealed a stronger co-ordination ability of the triphosphate as compared with that of the monophosphate. The comparison of the stability constants of the D-ribose and 2-deoxy-D-ribose complexes showed that more stable species were formed when neighbouring alcoholic hydroxy groups were available for the co-ordination of metal ions. The observed chemical shift changes of the 31P NMR resonances, as compared with those measured for the metal-free systems, demonstrated that the phosphate groups of the DNA fragment [5′-d(CGCGCG)2] chains act as binding sites for dimethyltin(IV)2+ between pH 4.5 and 7. The 1D and 2D 1H NMR spectra indicated that the base and sugar moieties do not participate in the coordination process under these conditions.

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