Potentiation of the glutamatergic synaptic input to rat locus coeruleus neurons by P2X7 receptors

Roghayeh Khakpay, Daniel Polster, L. Köles, Andrey Skorinkin, Bela Szabo, Kerstin Wirkner, Peter Illes

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Locus coeruleus (LC) neurons in a rat brain slice preparation were superfused with a Mg2+-free and bicuculline-containing external medium. Under these conditions, glutamatergic spontaneous excitatory postsynaptic currents (sEPSCs) were recorded by means of the whole-cell patch-clamp method. ATP, as well as its structural analogue 2-methylthio ATP (2-MeSATP), both caused transient inward currents, which were outlasted by an increase in the frequency but not the amplitude of the sEPSCs. PPADS, but not suramin or reactive blue 2 counteracted both effects of 2-MeSATP. By contrast, α,β-methylene ATP (α,β-meATP), UTP and BzATP did not cause an inward current response. Of these latter agonists, only BzATP slightly facilitated the sEPSC amplitude and strongly potentiated its frequency. PPADS and Brilliant Blue G, as well as fluorocitric acid and aminoadipic acid prevented the activity of BzATP. Furthermore, BzATP caused a similar facilitation of the miniature (m)EPSC (recorded in the presence of tetrodotoxin) and sEPSC frequencies (recorded in its absence). Eventually, capsaicin augmented the frequency of the sEPSCs in a capsazepine-, but not PPADS-antagonizable, manner. In conclusion, the stimulation of astrocytic P2X7 receptors appears to lead to the outflow of a signalling molecule, which presynaptically increases the spontaneous release of glutamate onto LC neurons from their afferent fibre tracts. It is suggested, that the two algogenic compounds ATP and capsaicin utilise separate receptor systems to potentiate the release of glutamate and in consequence to increase the excitability of LC neurons.

Original languageEnglish
Pages (from-to)349-359
Number of pages11
JournalPurinergic Signalling
Volume6
Issue number3
DOIs
Publication statusPublished - 2010

Fingerprint

Purinergic P2X7 Receptors
Locus Coeruleus
Excitatory Postsynaptic Potentials
Neurons
Cibacron Blue F 3GA
Adenosine Triphosphate
Capsaicin
Glutamic Acid
Suramin
Afferent Neurons
Uridine Triphosphate
Acids
Bicuculline
Tetrodotoxin
3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate
Brain
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
2-methylthio-ATP

Keywords

  • Adenosine 5′-triphosphate
  • Locus coeruleus
  • Miniature excitatory postsynaptic currents
  • P2X7 receptors
  • Presynaptic modulation
  • Spontaneous excitatory postsynaptic currents

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Potentiation of the glutamatergic synaptic input to rat locus coeruleus neurons by P2X7 receptors. / Khakpay, Roghayeh; Polster, Daniel; Köles, L.; Skorinkin, Andrey; Szabo, Bela; Wirkner, Kerstin; Illes, Peter.

In: Purinergic Signalling, Vol. 6, No. 3, 2010, p. 349-359.

Research output: Contribution to journalArticle

Khakpay, Roghayeh ; Polster, Daniel ; Köles, L. ; Skorinkin, Andrey ; Szabo, Bela ; Wirkner, Kerstin ; Illes, Peter. / Potentiation of the glutamatergic synaptic input to rat locus coeruleus neurons by P2X7 receptors. In: Purinergic Signalling. 2010 ; Vol. 6, No. 3. pp. 349-359.
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