Potentiation of photodynamic therapy with hypericin by mitomycin C in the radiation-induced fibrosarcoma-1 mouse tumor model

Bin Chen, Bissan Ahmed, Willy Landuyt, Yicheng Ni, R. Gáspár, Tania Roskams, Peter A M De Witte

Research output: Contribution to journalArticle

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Abstract

Hypericin, a polycyclic quinone obtained from plants of the genus Hypericum, has been shown to be a promising photosensitizer. We investigated the combination of hypericin-photodynamic therapy (PDT) and a bioreductive drug mitomycin C (MMC) in the present study. The radiation-induced fibrosarcoma-1 tumors were exposed to laser light (120 J/cm2 at 595 nm) 24 h after an intravenous injection of hypericin (1 mg/kg). Hypericin-PDT alone significantly decreased tumor perfusion and oxygen tension as demonstrated by India ink staining technique and OxyLite pO2 measurement, respectively. The in vivo-in vitro cell-survival assay revealed about 60% direct tumor cell killing immediately after PDT. No significant delayed tumor cell death was observed after PDT, which suggests that vascular damage does not contribute significantly to the overall tumor cell death. Injection of a 2.5 mg/kg dose of MMC 20 min before light application significantly decreased tumor cell survival and delayed tumor growth compared with PDT or MMC alone. No greater skin reaction was observed after the combination of MMC and PDT than after PDT alone. Our study demonstrates that combining hypericin-PDT with MMC can be effective in enhancing tumor response with little side effect.

Original languageEnglish
Pages (from-to)278-282
Number of pages5
JournalPhotochemistry and Photobiology
Volume78
Issue number3
DOIs
Publication statusPublished - Sep 1 2003

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Photodynamic therapy
Fibrosarcoma
Photochemotherapy
Mitomycin
mice
Tumors
therapy
tumors
Radiation
radiation
Neoplasms
Cell death
death
Cell Survival
Cell Death
oxygen tension
Cells
injection
Hypericum
Light

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Potentiation of photodynamic therapy with hypericin by mitomycin C in the radiation-induced fibrosarcoma-1 mouse tumor model. / Chen, Bin; Ahmed, Bissan; Landuyt, Willy; Ni, Yicheng; Gáspár, R.; Roskams, Tania; De Witte, Peter A M.

In: Photochemistry and Photobiology, Vol. 78, No. 3, 01.09.2003, p. 278-282.

Research output: Contribution to journalArticle

Chen, Bin ; Ahmed, Bissan ; Landuyt, Willy ; Ni, Yicheng ; Gáspár, R. ; Roskams, Tania ; De Witte, Peter A M. / Potentiation of photodynamic therapy with hypericin by mitomycin C in the radiation-induced fibrosarcoma-1 mouse tumor model. In: Photochemistry and Photobiology. 2003 ; Vol. 78, No. 3. pp. 278-282.
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