Potential urinary biomarkers of disease activity in Crohn's disease

Lajos Markó, Nóra Szigeti, Zoltán Szabó, Katalin Böddi, Anikó Takátsy, Andrea Ludány, T. Kőszegi, Gergó A. Molnár, I. Wittmann

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective. Measurement of the immunoreactive urinary albumin (ir-uAlb) concentration by immunological methods was found to be an effective method to identify disease activity in Crohn's disease (CD). Recently a size-exclusion (SE) high performance liquid chromatography (HPLC) method was developed to measure both ir-uAlb and non-immunoreactive urinary albumin (total, t-uAlb). We aimed to follow-up one of our CD patients with frequent remissions and exacerbation phases comparing the changes of disease activity parameters and the concentration of ir-uAlb and t-uAlb. The surprising results led us to perform measurements in greater depth. Material and methods. Concentration of ir-uAlb was measured by immunoturbidimetry (IT) and t-uAlb by SE-HPLC. Albumin peak of SE-HPLC was collected and applied to a reversed-phase (RP) HPLC and to gel-electrophoresis. Eluted peaks of RP-HPLC and identified bands of gel-electrophoresis were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Results. The concentration of t-uAlb was 15 times higher than that of the ir-uAlb during active state. The RP-HPLC and the gel-electrophoresis separation proved that albumin peak by size-exclusion consists of three different peaks. MALDI-TOF/MS measurements identified α1-acid-glycoprotein and Zn-α2-glycoprotein as major, and albumin as minor protein. Conclusions. Peak of albumin of SE-HPLC contains a significant amount of glycoprotein during the active phase of CD, which could not be detected in remission. Urinary α1-acid-glycoprotein and/or Zn-α2-glycoprotein could be an ideal disease activity biomarker of CD.

Original languageEnglish
Pages (from-to)1440-1448
Number of pages9
JournalScandinavian Journal of Gastroenterology
Volume45
Issue number12
DOIs
Publication statusPublished - Dec 2010

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Crohn Disease
Albumins
Biomarkers
High Pressure Liquid Chromatography
Glycoproteins
Electrophoresis
Gels
Mass Spectrometry
Lasers
Acids
Reverse-Phase Chromatography

Keywords

  • Alpha-1-acid-glycoprotein
  • Biomarker
  • Crohn's disease
  • Zinc-alpha-2- glycoprotein

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Potential urinary biomarkers of disease activity in Crohn's disease. / Markó, Lajos; Szigeti, Nóra; Szabó, Zoltán; Böddi, Katalin; Takátsy, Anikó; Ludány, Andrea; Kőszegi, T.; Molnár, Gergó A.; Wittmann, I.

In: Scandinavian Journal of Gastroenterology, Vol. 45, No. 12, 12.2010, p. 1440-1448.

Research output: Contribution to journalArticle

Markó, L, Szigeti, N, Szabó, Z, Böddi, K, Takátsy, A, Ludány, A, Kőszegi, T, Molnár, GA & Wittmann, I 2010, 'Potential urinary biomarkers of disease activity in Crohn's disease', Scandinavian Journal of Gastroenterology, vol. 45, no. 12, pp. 1440-1448. https://doi.org/10.3109/00365521.2010.505658
Markó L, Szigeti N, Szabó Z, Böddi K, Takátsy A, Ludány A et al. Potential urinary biomarkers of disease activity in Crohn's disease. Scandinavian Journal of Gastroenterology. 2010 Dec;45(12):1440-1448. https://doi.org/10.3109/00365521.2010.505658
Markó, Lajos ; Szigeti, Nóra ; Szabó, Zoltán ; Böddi, Katalin ; Takátsy, Anikó ; Ludány, Andrea ; Kőszegi, T. ; Molnár, Gergó A. ; Wittmann, I. / Potential urinary biomarkers of disease activity in Crohn's disease. In: Scandinavian Journal of Gastroenterology. 2010 ; Vol. 45, No. 12. pp. 1440-1448.
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abstract = "Objective. Measurement of the immunoreactive urinary albumin (ir-uAlb) concentration by immunological methods was found to be an effective method to identify disease activity in Crohn's disease (CD). Recently a size-exclusion (SE) high performance liquid chromatography (HPLC) method was developed to measure both ir-uAlb and non-immunoreactive urinary albumin (total, t-uAlb). We aimed to follow-up one of our CD patients with frequent remissions and exacerbation phases comparing the changes of disease activity parameters and the concentration of ir-uAlb and t-uAlb. The surprising results led us to perform measurements in greater depth. Material and methods. Concentration of ir-uAlb was measured by immunoturbidimetry (IT) and t-uAlb by SE-HPLC. Albumin peak of SE-HPLC was collected and applied to a reversed-phase (RP) HPLC and to gel-electrophoresis. Eluted peaks of RP-HPLC and identified bands of gel-electrophoresis were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Results. The concentration of t-uAlb was 15 times higher than that of the ir-uAlb during active state. The RP-HPLC and the gel-electrophoresis separation proved that albumin peak by size-exclusion consists of three different peaks. MALDI-TOF/MS measurements identified α1-acid-glycoprotein and Zn-α2-glycoprotein as major, and albumin as minor protein. Conclusions. Peak of albumin of SE-HPLC contains a significant amount of glycoprotein during the active phase of CD, which could not be detected in remission. Urinary α1-acid-glycoprotein and/or Zn-α2-glycoprotein could be an ideal disease activity biomarker of CD.",
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AU - Takátsy, Anikó

AU - Ludány, Andrea

AU - Kőszegi, T.

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N2 - Objective. Measurement of the immunoreactive urinary albumin (ir-uAlb) concentration by immunological methods was found to be an effective method to identify disease activity in Crohn's disease (CD). Recently a size-exclusion (SE) high performance liquid chromatography (HPLC) method was developed to measure both ir-uAlb and non-immunoreactive urinary albumin (total, t-uAlb). We aimed to follow-up one of our CD patients with frequent remissions and exacerbation phases comparing the changes of disease activity parameters and the concentration of ir-uAlb and t-uAlb. The surprising results led us to perform measurements in greater depth. Material and methods. Concentration of ir-uAlb was measured by immunoturbidimetry (IT) and t-uAlb by SE-HPLC. Albumin peak of SE-HPLC was collected and applied to a reversed-phase (RP) HPLC and to gel-electrophoresis. Eluted peaks of RP-HPLC and identified bands of gel-electrophoresis were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Results. The concentration of t-uAlb was 15 times higher than that of the ir-uAlb during active state. The RP-HPLC and the gel-electrophoresis separation proved that albumin peak by size-exclusion consists of three different peaks. MALDI-TOF/MS measurements identified α1-acid-glycoprotein and Zn-α2-glycoprotein as major, and albumin as minor protein. Conclusions. Peak of albumin of SE-HPLC contains a significant amount of glycoprotein during the active phase of CD, which could not be detected in remission. Urinary α1-acid-glycoprotein and/or Zn-α2-glycoprotein could be an ideal disease activity biomarker of CD.

AB - Objective. Measurement of the immunoreactive urinary albumin (ir-uAlb) concentration by immunological methods was found to be an effective method to identify disease activity in Crohn's disease (CD). Recently a size-exclusion (SE) high performance liquid chromatography (HPLC) method was developed to measure both ir-uAlb and non-immunoreactive urinary albumin (total, t-uAlb). We aimed to follow-up one of our CD patients with frequent remissions and exacerbation phases comparing the changes of disease activity parameters and the concentration of ir-uAlb and t-uAlb. The surprising results led us to perform measurements in greater depth. Material and methods. Concentration of ir-uAlb was measured by immunoturbidimetry (IT) and t-uAlb by SE-HPLC. Albumin peak of SE-HPLC was collected and applied to a reversed-phase (RP) HPLC and to gel-electrophoresis. Eluted peaks of RP-HPLC and identified bands of gel-electrophoresis were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Results. The concentration of t-uAlb was 15 times higher than that of the ir-uAlb during active state. The RP-HPLC and the gel-electrophoresis separation proved that albumin peak by size-exclusion consists of three different peaks. MALDI-TOF/MS measurements identified α1-acid-glycoprotein and Zn-α2-glycoprotein as major, and albumin as minor protein. Conclusions. Peak of albumin of SE-HPLC contains a significant amount of glycoprotein during the active phase of CD, which could not be detected in remission. Urinary α1-acid-glycoprotein and/or Zn-α2-glycoprotein could be an ideal disease activity biomarker of CD.

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