Externalizációs problémákkal küzdo serdülo fiúk nyál biomarkereinek azonosítása és ezek genetikai befolyásoltsága

Translated title of the contribution: Potential salivary biomarkers and their genetic effects in a pilot study of adolescent boys with externalizing problems

Nóra Angyal, J. Halász, Gergely Mészáros, Judit Krisztina Kovács, Emese Kruk, Z. Nemoda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aims: Beside the well-known stress response marker cortisol, salivary alpha-amylase is receiving increasing attention. Numerous studies have investigated the potential biomarker properties of cortisol mirroring abnormal hypothalamic-pituitary-adrenal axis activity in connection to both internalizing and externalizing behavior problems. The other major physiological system involved in stress reactivity, the sympathetic nervous system activity can be also measured by the surrogate marker of salivary alpha-amylase. Most of the studies applied a stressful situation to obtain inter-individual differences in stress-reactivity, although differences in the baseline level of cortisol have been also shown in relation to externalizing problems. To test the relevance of another (easier) biomarker, we selected to study baseline circadian salivary cortisol and alpha-amylase levels among adolescent boys with externalizing problems. Methods: Saliva samples were collected at 3 time-points (morning, noon, evening) during 3 consecutive days from 37 inpatient boys (mean age 12.4±1.0). Cortisol and alpha-amylase levels were measured by enzyme-linked immunosorbent and kinetic enzyme assays, respectively. Genetic variants in the hypothalamic-pituitary-adrenal axis and the norepinephrine transporter or catecholamine metabolizing enzymes were tested for potential moderating effects at these salivary biomarkers. Results: Saliva cortisol showed the classical diurnal fluctuation in boys with externalizing problems (possibly from a lower morning level), but it was not modified by the presence of either conduct, oppositional defiant or attention-deficit/hyperactivity disorder. The diurnal fluctuation of the salivary alpha-amylase levels was also typical, but the presence of conduct disorder was associated with significantly lower alpha-amylase activity (p=0.024) among boys with externalizing problems. The catechol-O-methyltransferase Val158Met (rs4680) polymorphism had an additional effect on salivary alpha-amylase: boys with homozygote genotypes had lower alpha-amylase activity at all 3 time-points compared to Val/Met heterozygotes (p=0.045). Conclusions: Our preliminary data suggest that salivary alpha-amylase might be used to further characterize subgroups within externalizing problems, however, this biomarker might be modified by certain genetic polymorphisms.

Original languageHungarian
Pages (from-to)173-179
Number of pages7
JournalNeuropsychopharmacologia Hungarica
Volume18
Issue number4
Publication statusPublished - Dec 1 2016

Fingerprint

Salivary alpha-Amylases
Hydrocortisone
Biomarkers
alpha-Amylases
Saliva
Norepinephrine Plasma Membrane Transport Proteins
Catechol O-Methyltransferase
Conduct Disorder
Sympathetic Nervous System
Homozygote
Genetic Polymorphisms
Enzymes
Attention Deficit Disorder with Hyperactivity
Heterozygote
Individuality
Catecholamines
Inpatients
Enzyme-Linked Immunosorbent Assay
Genotype

ASJC Scopus subject areas

  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Clinical Neurology

Cite this

Externalizációs problémákkal küzdo serdülo fiúk nyál biomarkereinek azonosítása és ezek genetikai befolyásoltsága. / Angyal, Nóra; Halász, J.; Mészáros, Gergely; Kovács, Judit Krisztina; Kruk, Emese; Nemoda, Z.

In: Neuropsychopharmacologia Hungarica, Vol. 18, No. 4, 01.12.2016, p. 173-179.

Research output: Contribution to journalArticle

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abstract = "Aims: Beside the well-known stress response marker cortisol, salivary alpha-amylase is receiving increasing attention. Numerous studies have investigated the potential biomarker properties of cortisol mirroring abnormal hypothalamic-pituitary-adrenal axis activity in connection to both internalizing and externalizing behavior problems. The other major physiological system involved in stress reactivity, the sympathetic nervous system activity can be also measured by the surrogate marker of salivary alpha-amylase. Most of the studies applied a stressful situation to obtain inter-individual differences in stress-reactivity, although differences in the baseline level of cortisol have been also shown in relation to externalizing problems. To test the relevance of another (easier) biomarker, we selected to study baseline circadian salivary cortisol and alpha-amylase levels among adolescent boys with externalizing problems. Methods: Saliva samples were collected at 3 time-points (morning, noon, evening) during 3 consecutive days from 37 inpatient boys (mean age 12.4±1.0). Cortisol and alpha-amylase levels were measured by enzyme-linked immunosorbent and kinetic enzyme assays, respectively. Genetic variants in the hypothalamic-pituitary-adrenal axis and the norepinephrine transporter or catecholamine metabolizing enzymes were tested for potential moderating effects at these salivary biomarkers. Results: Saliva cortisol showed the classical diurnal fluctuation in boys with externalizing problems (possibly from a lower morning level), but it was not modified by the presence of either conduct, oppositional defiant or attention-deficit/hyperactivity disorder. The diurnal fluctuation of the salivary alpha-amylase levels was also typical, but the presence of conduct disorder was associated with significantly lower alpha-amylase activity (p=0.024) among boys with externalizing problems. The catechol-O-methyltransferase Val158Met (rs4680) polymorphism had an additional effect on salivary alpha-amylase: boys with homozygote genotypes had lower alpha-amylase activity at all 3 time-points compared to Val/Met heterozygotes (p=0.045). Conclusions: Our preliminary data suggest that salivary alpha-amylase might be used to further characterize subgroups within externalizing problems, however, this biomarker might be modified by certain genetic polymorphisms.",
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AU - Halász, J.

AU - Mészáros, Gergely

AU - Kovács, Judit Krisztina

AU - Kruk, Emese

AU - Nemoda, Z.

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