Potential role of formaldehyde in the mechanism of action of ascorbigens on the basis of BioArena studies

G. Kátay, P. Ott, Edit Kátay, Donát Magyar, E. Tyihák

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of ascorbigen and 1′-methylascorbigen as a model compound pair was studied on the phytopathogenic bacterium Pseudomonas savastanoi pv. phaseolicola in the BioArena experimental system after overpressured layer chromatography. Results showed a characteristic, strong antibacterial effect of 1′-methylascorbigen and weak effect of ascorbigen present on the adsorbent layer as chromatographic spot. Addition of formaldehyde capture compounds (L-arginine, glutathione, dimedone) partially or totally reduced the antibacterial effect of 1′-methylascorbigen and ascorbigen. On adding Cu(II) ions - which mobilize and coordinate formaldehyde - to the culture medium, the antibacterial effect of both compounds became stronger. It is supposed that the weak antibacterial effect of ascorbigen may have originated from the 1′-methylascorbigen formed in situ on the adsorbent layer by partial enzymatic methylation of ascorbigen.

Original languageEnglish
Pages (from-to)412-418
Number of pages7
JournalBiomedical Chromatography
Volume23
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Formaldehyde
Adsorbents
Methylation
Chromatography
Pseudomonas
Glutathione
Culture Media
Arginine
Bacteria
ascorbigen
Ions
1'-methylascorbigen

Keywords

  • Ascorbigen
  • BioArena system
  • Formaldehyde
  • Mechanism of action
  • Methylascorbigen
  • Overpressured layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Pharmacology

Cite this

Potential role of formaldehyde in the mechanism of action of ascorbigens on the basis of BioArena studies. / Kátay, G.; Ott, P.; Kátay, Edit; Magyar, Donát; Tyihák, E.

In: Biomedical Chromatography, Vol. 23, No. 4, 2009, p. 412-418.

Research output: Contribution to journalArticle

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