Potent effect of interleukin-1β to evoke ATP and adenosine release from rat hippocampal slices

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41 Citations (Scopus)

Abstract

In this study the effect of IL-1β on [3H]purine release from rat hippocampal slices was explored. IL-1β (3×10 -18-3×10-14 M) concentration-dependently elevated the basal [3H]purine efflux, and this effect was reversed by the selective IL-1RI receptor antagonist IL-1ra (10-12 M). HPLC analysis revealed that the amount of [3H]ATP and [3H]adenosine significantly increased in the effluent in response to IL-1β. The sodium channel inhibitor tetrodotoxin, the NMDA and non-NMDA receptor antagonists D(-)-2-amino-5-phosphonopentanoic acid (AP-5) plus 6-cyano-7-nitroquinoxaline-2, 3-dione-disodium (CNQX) almost completely abolished IL-1β-evoked [ 3H]purine release. The effect of IL-1β on [3H]purine efflux was also prevented by the p38 MAP kinase inhibitor SB 203580, by the nucleoside transport inhibitor nitrobenzyl-thioinosine (NBTI) and by low temperature (4°C). In summary IL-1β triggers a transporter mediated [3H]purine efflux in the hippocampus which is conveyed by glutamate receptor activation and the p38 MAP kinase pathway, and could serve as a mediator of IL-1β-induced synaptic depression.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJournal of Neuroimmunology
Volume151
Issue number1-2
DOIs
Publication statusPublished - Jun 1 2004

Keywords

  • Adenosine
  • Hippocampus
  • IL-1β
  • Release
  • p38MAP kinase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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