Potassium channels regulate hypo-osmotic shock-induced motility of common carp (Cyprinus carpio) sperm

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Abstract

The osmolality and composition of fish seminal plasma usually suppress sperm motility in the testis and sperm duct. Change in the osmolality of the environment at spawning activates flagellar motion of the sperm tail. The effect of inhibitors of anion and cation channels on active motion was investigated by a microscopic motility test. While sodium channel inhibitors (amiloride and tetrodotoxin) as well as anion channel inhibitors (DIDS and ethacrynic acid) did not affect motility, potassium channel inhibitors (4-AP, quinine, veratrine, verapamil) decreased the duration of flagellar motion or abolished motility completely. Potassium channel inhibitors exerted an effect on sperm motility depending on the dose and length of incubation. The voltage-activated K+ blocker 4-aminopiridine proved to be the most potent inhibitor. Its effect was completely reversible, indicating that the functional state of potassium channels is essential to the activation mechanism of sperm. Intracellular pH of the spermatozoa was measured by a fluorescent flow cytometric technique. Hypo-osmotic shock induced intracellular alkalinization of carp sperm. Increase in pHi was 0.15 pH units. In the presence of 4-AP the measured amplitude and kinetics of the pHi change was the same as in the absence of this inhibitor, arguing against a regulatory role of intracellular pH change.

Original languageEnglish
Pages (from-to)123-128
Number of pages6
JournalAquaculture
Volume129
Issue number1-4
DOIs
Publication statusPublished - 1995

Fingerprint

potassium channels
motility
osmotic stress
Cyprinus carpio
sperm
inhibitor
potassium
spermatozoa
osmolality
sperm motility
anions
ethacrynic acid
alkalinization
tetrodotoxin
quinine
anion
verapamil
sodium channels
seminal plasma
carp

Keywords

  • Anion channel inhibitors
  • Cyprinus carpio
  • Hypoosmotic shock
  • Intracellular pH
  • Potassium channel inhibitors
  • Sperm motility

ASJC Scopus subject areas

  • Aquatic Science

Cite this

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title = "Potassium channels regulate hypo-osmotic shock-induced motility of common carp (Cyprinus carpio) sperm",
abstract = "The osmolality and composition of fish seminal plasma usually suppress sperm motility in the testis and sperm duct. Change in the osmolality of the environment at spawning activates flagellar motion of the sperm tail. The effect of inhibitors of anion and cation channels on active motion was investigated by a microscopic motility test. While sodium channel inhibitors (amiloride and tetrodotoxin) as well as anion channel inhibitors (DIDS and ethacrynic acid) did not affect motility, potassium channel inhibitors (4-AP, quinine, veratrine, verapamil) decreased the duration of flagellar motion or abolished motility completely. Potassium channel inhibitors exerted an effect on sperm motility depending on the dose and length of incubation. The voltage-activated K+ blocker 4-aminopiridine proved to be the most potent inhibitor. Its effect was completely reversible, indicating that the functional state of potassium channels is essential to the activation mechanism of sperm. Intracellular pH of the spermatozoa was measured by a fluorescent flow cytometric technique. Hypo-osmotic shock induced intracellular alkalinization of carp sperm. Increase in pHi was 0.15 pH units. In the presence of 4-AP the measured amplitude and kinetics of the pHi change was the same as in the absence of this inhibitor, arguing against a regulatory role of intracellular pH change.",
keywords = "Anion channel inhibitors, Cyprinus carpio, Hypoosmotic shock, Intracellular pH, Potassium channel inhibitors, Sperm motility",
author = "Z. Krasznai and T. M{\'a}ri{\'a}n and L. Balkay and R. G{\'a}sp{\'a}r and L. Tr{\'o}n",
year = "1995",
doi = "10.1016/0044-8486(94)00234-F",
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T1 - Potassium channels regulate hypo-osmotic shock-induced motility of common carp (Cyprinus carpio) sperm

AU - Krasznai, Z.

AU - Márián, T.

AU - Balkay, L.

AU - Gáspár, R.

AU - Trón, L.

PY - 1995

Y1 - 1995

N2 - The osmolality and composition of fish seminal plasma usually suppress sperm motility in the testis and sperm duct. Change in the osmolality of the environment at spawning activates flagellar motion of the sperm tail. The effect of inhibitors of anion and cation channels on active motion was investigated by a microscopic motility test. While sodium channel inhibitors (amiloride and tetrodotoxin) as well as anion channel inhibitors (DIDS and ethacrynic acid) did not affect motility, potassium channel inhibitors (4-AP, quinine, veratrine, verapamil) decreased the duration of flagellar motion or abolished motility completely. Potassium channel inhibitors exerted an effect on sperm motility depending on the dose and length of incubation. The voltage-activated K+ blocker 4-aminopiridine proved to be the most potent inhibitor. Its effect was completely reversible, indicating that the functional state of potassium channels is essential to the activation mechanism of sperm. Intracellular pH of the spermatozoa was measured by a fluorescent flow cytometric technique. Hypo-osmotic shock induced intracellular alkalinization of carp sperm. Increase in pHi was 0.15 pH units. In the presence of 4-AP the measured amplitude and kinetics of the pHi change was the same as in the absence of this inhibitor, arguing against a regulatory role of intracellular pH change.

AB - The osmolality and composition of fish seminal plasma usually suppress sperm motility in the testis and sperm duct. Change in the osmolality of the environment at spawning activates flagellar motion of the sperm tail. The effect of inhibitors of anion and cation channels on active motion was investigated by a microscopic motility test. While sodium channel inhibitors (amiloride and tetrodotoxin) as well as anion channel inhibitors (DIDS and ethacrynic acid) did not affect motility, potassium channel inhibitors (4-AP, quinine, veratrine, verapamil) decreased the duration of flagellar motion or abolished motility completely. Potassium channel inhibitors exerted an effect on sperm motility depending on the dose and length of incubation. The voltage-activated K+ blocker 4-aminopiridine proved to be the most potent inhibitor. Its effect was completely reversible, indicating that the functional state of potassium channels is essential to the activation mechanism of sperm. Intracellular pH of the spermatozoa was measured by a fluorescent flow cytometric technique. Hypo-osmotic shock induced intracellular alkalinization of carp sperm. Increase in pHi was 0.15 pH units. In the presence of 4-AP the measured amplitude and kinetics of the pHi change was the same as in the absence of this inhibitor, arguing against a regulatory role of intracellular pH change.

KW - Anion channel inhibitors

KW - Cyprinus carpio

KW - Hypoosmotic shock

KW - Intracellular pH

KW - Potassium channel inhibitors

KW - Sperm motility

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