Postmortem small babesia-like morphology of Babesia canis - Short communication

Zoltán Demeter, Elena Palade, Éva Balogh, Csaba Jakab, R. Farkas, Balázs Tánczos, S. Hornok

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Here we report a case of canine babesiosis with unusual morphology of the causative agent. A male, seven-week-old Labrador retriever puppy, exhibiting severe anaemia and haemoglobinuria, was presented at the Clinic of Internal Medicine in February 2011. The puppy was euthanised. The most relevant pathological changes were icterus, severe splenomegaly, generalised lymphadenopathy and haemoglobin nephrosis. Samples were collected from various organs for histology within one hour post mortem. Impression smears were also prepared from the spleen after overnight storage at 4 °C. Tissue sections and smears showed the presence of multiple, coccoid intraerythrocytic bodies that measured 1-2 μm and resembled small babesiae. No large piroplasms were seen. DNA was extracted from the spleen, and a conventional PCR was performed for the amplification of a 450-bp region of the 18S rRNA gene of piroplasms. The causative agent was identified as Babesia canis canis, with 99% sequence identity to other European isolates. Sequence identity to B. gibsoni was only 91%. This is the first account to verify that the morphology of the large canine piroplasm, B. canis, can be uniformly small babesia-like post mortem or following the storage of tissue samples.

Original languageEnglish
Pages (from-to)427-432
Number of pages6
JournalActa Veterinaria Hungarica
Volume59
Issue number4
DOIs
Publication statusPublished - Dec 1 2011

Fingerprint

Babesia canis
Piroplasmida
Babesia
animal communication
Canidae
Spleen
puppies
Hemoglobinuria
Babesiosis
Nephrosis
Newfoundland and Labrador
Splenomegaly
spleen
Internal Medicine
Jaundice
rRNA Genes
Babesia gibsoni
hemoglobinuria
nephrosis
Anemia

Keywords

  • Babesia canis
  • Babesia gibsoni
  • Morphology
  • Small babesia
  • Viscera

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Postmortem small babesia-like morphology of Babesia canis - Short communication. / Demeter, Zoltán; Palade, Elena; Balogh, Éva; Jakab, Csaba; Farkas, R.; Tánczos, Balázs; Hornok, S.

In: Acta Veterinaria Hungarica, Vol. 59, No. 4, 01.12.2011, p. 427-432.

Research output: Contribution to journalArticle

Demeter, Zoltán ; Palade, Elena ; Balogh, Éva ; Jakab, Csaba ; Farkas, R. ; Tánczos, Balázs ; Hornok, S. / Postmortem small babesia-like morphology of Babesia canis - Short communication. In: Acta Veterinaria Hungarica. 2011 ; Vol. 59, No. 4. pp. 427-432.
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