Postmenopausal expression changes of immune system-related genes in human bone tissue

J. Kósa, Bernadett Balla, János Kiss, J. Podaní, I. Takács, Áron Lazáry, Zsolt Nagy, Krisztián Bácsi, Attila Karsai, G. Speer, P. Lakatos

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Introduction: The molecular and cellular interactions between the immune system and bone tissue have been established. Sex hormone deficiency after menopause has multifunctional role by influencing growth, differentiation, and metabolism of the skeletal and the immune system. Discussion: We have used nonparametric and multidimensional expression pattern analyses to determine significantly changed mRNA profile of immune system-associated genes in postmenopausal (POST) and premenopausal (PRE) nonosteoporotic bone. Ten bone tissue samples from POST patients and six bone tissue samples from PRE women were examined in our study. The transcription differences of the selected 50 genes were analyzed in TaqMan probe-based quantitative real-time reverse transcriptase polymerase chain reaction system. Mann-Whitney test indicated significantly downregulated transcription activity of three genes (CD14, HLA-A/MHCI, ITGAM/CD11b) and upregulated expression of six genes (C3, CD86/B7-2, IL-10, IL-6, TGFB3, TNFSF11/RANKL) in postmenopausal bone. According to the canonical variate analysis results, the groups of POST and PRE women are separable by genes coding for cytokines, costimulator molecules, and cell surface receptors involved in antigen presentation and T cell stimulation processes which have high discriminatory power. Based on a complex gene expression pattern analysis of human bone tissue, we could distinguish POST and PRE states from an immunological aspect. Our data might provide further insight into the changes of the intersystem crosstalk between immune and skeletal homeostasis, as well as local immune response in the altered microenvironment of postmenopausal bone.

Original languageEnglish
Pages (from-to)761-768
Number of pages8
JournalJournal of Clinical Immunology
Volume29
Issue number6
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Immune System
Bone and Bones
Genes
Transforming Growth Factor beta3
HLA-A Antigens
Antigen Presentation
Gonadal Steroid Hormones
Cell Surface Receptors
Gene Expression Profiling
Menopause
Reverse Transcriptase Polymerase Chain Reaction
Interleukin-10
Real-Time Polymerase Chain Reaction
Interleukin-6
Homeostasis
Down-Regulation
Cytokines
T-Lymphocytes
Gene Expression
Messenger RNA

Keywords

  • Human bone tissue
  • Immune-related genes
  • Menopause
  • Transcription pattern

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Postmenopausal expression changes of immune system-related genes in human bone tissue. / Kósa, J.; Balla, Bernadett; Kiss, János; Podaní, J.; Takács, I.; Lazáry, Áron; Nagy, Zsolt; Bácsi, Krisztián; Karsai, Attila; Speer, G.; Lakatos, P.

In: Journal of Clinical Immunology, Vol. 29, No. 6, 11.2009, p. 761-768.

Research output: Contribution to journalArticle

Kósa, J. ; Balla, Bernadett ; Kiss, János ; Podaní, J. ; Takács, I. ; Lazáry, Áron ; Nagy, Zsolt ; Bácsi, Krisztián ; Karsai, Attila ; Speer, G. ; Lakatos, P. / Postmenopausal expression changes of immune system-related genes in human bone tissue. In: Journal of Clinical Immunology. 2009 ; Vol. 29, No. 6. pp. 761-768.
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AU - Lazáry, Áron

AU - Nagy, Zsolt

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