A menopausa hatása az immunrendszer muködését szabályozó gének transzkriptomikai változá saira human csontszövetben

Translated title of the contribution: Postmenopausal changes of immune system-related genes expression in human bone tissue

Bernadett Balla, János Kósa P, János Kiss, J. Podaní, I. Takács, Áron Lazary, Krisztián Bácsi, Zsolt Nagy, G. Speer, P. Lakatos

Research output: Contribution to journalArticle

Abstract

The molecular and cellular interactions between the immune system and bone tissue have been established. Sex hormone deficiency at the time of menopause has multifunctional role by influencing growth, differentiation and metabolism of the skeletal and the immune system. We have used non-parametric and multidimensional expression pattern analyses to determine significantly changed mRNA profile of immune system-associated genes in postmenopausal (POST) and premenopausal (PRE) non-osteoporotic bone. MATERIALS AND METHODS - Ten bone tissue samples from POST patients and six bone tissue samples from PRE women were examined in our study. The transcription differences of selected 50 genes were analyzed in Taqman probe-based quantitative real-time RT-PCR system. RESULTS - Mann-Whitney test indicated significantly down-regulated transcription activity of 3 genes (CD14, HLA-A, ITGAM/CD11b) and upregulated gene expression of 6 genes (C3, CD86, IL-10, IL-6, TGFB3, TNFSF11/RANKL) in POST bone. According to the canonical variates analysis results, the groups of postmenopausal and premenopausal women are separable by genes coding for cytokines, co-stimulators and cell surface receptors affected in antigen presentation and T cell stimulation which have high discriminatory power. CONCLUSIONS - Based on a complex gene expression patterns in human bone cells, we could distinguish POST and PRE states from an immunological aspect. Our data might provide further insight into the changes of the intersystem crosstalk between immune and skeletal system, as well as local immune response in the altered microenvironment of POST bone.

Original languageHungarian
Pages (from-to)667-673
Number of pages7
JournalLege Artis Medicinae
Volume20
Issue number10
Publication statusPublished - Oct 2010

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Immune System
Gene Expression
Bone and Bones
Genes
Transforming Growth Factor beta3
HLA-A Antigens
Antigen Presentation
Gonadal Steroid Hormones
Cell Surface Receptors
Menopause
Interleukin-10
Real-Time Polymerase Chain Reaction
Interleukin-6
Cytokines
T-Lymphocytes
Messenger RNA
Growth

ASJC Scopus subject areas

  • Medicine(all)

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A menopausa hatása az immunrendszer muködését szabályozó gének transzkriptomikai változá saira human csontszövetben. / Balla, Bernadett; Kósa P, János; Kiss, János; Podaní, J.; Takács, I.; Lazary, Áron; Bácsi, Krisztián; Nagy, Zsolt; Speer, G.; Lakatos, P.

In: Lege Artis Medicinae, Vol. 20, No. 10, 10.2010, p. 667-673.

Research output: Contribution to journalArticle

Balla, Bernadett ; Kósa P, János ; Kiss, János ; Podaní, J. ; Takács, I. ; Lazary, Áron ; Bácsi, Krisztián ; Nagy, Zsolt ; Speer, G. ; Lakatos, P. / A menopausa hatása az immunrendszer muködését szabályozó gének transzkriptomikai változá saira human csontszövetben. In: Lege Artis Medicinae. 2010 ; Vol. 20, No. 10. pp. 667-673.
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abstract = "The molecular and cellular interactions between the immune system and bone tissue have been established. Sex hormone deficiency at the time of menopause has multifunctional role by influencing growth, differentiation and metabolism of the skeletal and the immune system. We have used non-parametric and multidimensional expression pattern analyses to determine significantly changed mRNA profile of immune system-associated genes in postmenopausal (POST) and premenopausal (PRE) non-osteoporotic bone. MATERIALS AND METHODS - Ten bone tissue samples from POST patients and six bone tissue samples from PRE women were examined in our study. The transcription differences of selected 50 genes were analyzed in Taqman probe-based quantitative real-time RT-PCR system. RESULTS - Mann-Whitney test indicated significantly down-regulated transcription activity of 3 genes (CD14, HLA-A, ITGAM/CD11b) and upregulated gene expression of 6 genes (C3, CD86, IL-10, IL-6, TGFB3, TNFSF11/RANKL) in POST bone. According to the canonical variates analysis results, the groups of postmenopausal and premenopausal women are separable by genes coding for cytokines, co-stimulators and cell surface receptors affected in antigen presentation and T cell stimulation which have high discriminatory power. CONCLUSIONS - Based on a complex gene expression patterns in human bone cells, we could distinguish POST and PRE states from an immunological aspect. Our data might provide further insight into the changes of the intersystem crosstalk between immune and skeletal system, as well as local immune response in the altered microenvironment of POST bone.",
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AU - Balla, Bernadett

AU - Kósa P, János

AU - Kiss, János

AU - Podaní, J.

AU - Takács, I.

AU - Lazary, Áron

AU - Bácsi, Krisztián

AU - Nagy, Zsolt

AU - Speer, G.

AU - Lakatos, P.

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AB - The molecular and cellular interactions between the immune system and bone tissue have been established. Sex hormone deficiency at the time of menopause has multifunctional role by influencing growth, differentiation and metabolism of the skeletal and the immune system. We have used non-parametric and multidimensional expression pattern analyses to determine significantly changed mRNA profile of immune system-associated genes in postmenopausal (POST) and premenopausal (PRE) non-osteoporotic bone. MATERIALS AND METHODS - Ten bone tissue samples from POST patients and six bone tissue samples from PRE women were examined in our study. The transcription differences of selected 50 genes were analyzed in Taqman probe-based quantitative real-time RT-PCR system. RESULTS - Mann-Whitney test indicated significantly down-regulated transcription activity of 3 genes (CD14, HLA-A, ITGAM/CD11b) and upregulated gene expression of 6 genes (C3, CD86, IL-10, IL-6, TGFB3, TNFSF11/RANKL) in POST bone. According to the canonical variates analysis results, the groups of postmenopausal and premenopausal women are separable by genes coding for cytokines, co-stimulators and cell surface receptors affected in antigen presentation and T cell stimulation which have high discriminatory power. CONCLUSIONS - Based on a complex gene expression patterns in human bone cells, we could distinguish POST and PRE states from an immunological aspect. Our data might provide further insight into the changes of the intersystem crosstalk between immune and skeletal system, as well as local immune response in the altered microenvironment of POST bone.

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