Postischemic calmodulin gene expression in the rat hippocampus

Arpad Palfi, Janos Aurel Simonka, Monika Pataricza, Peter Tekulics, I. Leprán, J. Papp, K. Gulya

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The hippocampal calmodulin (CaM) gene expression was determined in the rat after transient forebrain ischemia. Ischemia was induced by the 4-vessel occlusion model, and the hybridized mRNA copy numbers corresponding to the 3 CaM genes detected by phosphorimaging were determined by quantitative in situ hybridization 24 h post-insult. A small, but significant upregulation (by 8.8%) of the CaM I gene was observed in the CA1 pyramidal cell layer, whereas other regions exhibited a maintained or slightly decreased CaM gene expression. The CaM mRNA levels decreased most markedly (by 10-15%) in the hippocampal molecular layers. No consistent correlation was found between the ischemic vulnerability and the CaM gene expression pattern. The results indicate that the induction of delayed neuronal death is not incidental to the transcriptional activation of the CaM genes in the ischemic rat hippocampus in vivo. As the calcium-bound CaM content increases during the ischemic insult, downregulation of the CaM gene expression could be a homeostatic response aimed at maintaining the intracellular level of the active CaM pool.

Original languageEnglish
Pages (from-to)2373-2381
Number of pages9
JournalLife Sciences
Volume68
Issue number21
DOIs
Publication statusPublished - Apr 13 2001

Fingerprint

Calmodulin
Gene expression
Rats
Hippocampus
Gene Expression
Genes
Ischemia
Hippocampal CA1 Region
Messenger RNA
Prosencephalon
Transcriptional Activation
In Situ Hybridization
Up-Regulation
Down-Regulation
Chemical activation
Cells
Calcium

Keywords

  • 4-Vessel occlusion model
  • Calmodulin gene expression
  • Forebrain ischemia
  • Hippocampus
  • Quantitative in situ hybridization
  • Rat

ASJC Scopus subject areas

  • Pharmacology

Cite this

Postischemic calmodulin gene expression in the rat hippocampus. / Palfi, Arpad; Simonka, Janos Aurel; Pataricza, Monika; Tekulics, Peter; Leprán, I.; Papp, J.; Gulya, K.

In: Life Sciences, Vol. 68, No. 21, 13.04.2001, p. 2373-2381.

Research output: Contribution to journalArticle

Palfi, Arpad ; Simonka, Janos Aurel ; Pataricza, Monika ; Tekulics, Peter ; Leprán, I. ; Papp, J. ; Gulya, K. / Postischemic calmodulin gene expression in the rat hippocampus. In: Life Sciences. 2001 ; Vol. 68, No. 21. pp. 2373-2381.
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AU - Papp, J.

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