Postconditioning of the small intestine

Which is the most effective algorithm in a rat model?

Oliver Rosero, Peter Onody, Rita Stangl, Zsolt Turoczi, Andras Fulop, David Garbaisz, G. Lotz, Laszlo Harsanyi, Attila Szijarto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background Mesenteric ischemia is a serious clinical condition requiring immediate surgical intervention. The unavoidable ischemic-reperfusion (IR) injury may be ameliorated using the appropriate postconditioning protocol. The aim of the present study was to investigate the optimal postconditioning algorithm in a rat model of intestinal ischemic-reperfusion injury. Materials and methods Male Wistar rats were randomized into five groups (n = 10), one sham-operated, one IR, and three postconditioned groups, each with different protocols. The animals were subjected to 60 min of mesenteric ischemia, followed by 60 min of reperfusion. Postconditioning was applied at the onset of reperfusion using three different algorithms. Arterial pressure and mucosal microcirculation were monitored throughout the experiment. Mesenteric pH was determined at the early phase of reperfusion. Blood and tissue samples were taken at the end of reperfusion for histologic evaluation, serum lactate dehydrogenase, serum creatine kinase, serum tumor necrosis factor-α, serum interleukin-6, detailed mucosal antioxidant, and scavenger capacity assays. Results The shorter and intermediate length cycles of postconditioning enhanced mucosal microcirculation and redox state and significantly delayed the normalization of mesenteric pH. Furthermore, milder histopathologic lesions and lower concentrations of serum necroenzymes and proinflammatory cytokines were detected compared with the IR group. The protective effect of postconditioning using longer cycles could only be seen in a tendentious manner. Conclusions In a rat model of intestinal ischemia-reperfusion, the shorter and intermediate length cycles of postconditioning proved to be more effective than the use of longer cycles.

Original languageEnglish
Pages (from-to)427-437
Number of pages11
JournalJournal of Surgical Research
Volume187
Issue number2
DOIs
Publication statusPublished - Apr 2014

Fingerprint

Small Intestine
Reperfusion
Serum
Microcirculation
Reperfusion Injury
Creatine Kinase
L-Lactate Dehydrogenase
Oxidation-Reduction
Wistar Rats
Interleukin-6
Arterial Pressure
Ischemia
Tumor Necrosis Factor-alpha
Antioxidants
Cytokines

Keywords

  • Intestinal ischemia
  • Mucosal microcirculation
  • Postconditioning algorithms
  • Rat
  • Reperfusion injury

ASJC Scopus subject areas

  • Surgery

Cite this

Rosero, O., Onody, P., Stangl, R., Turoczi, Z., Fulop, A., Garbaisz, D., ... Szijarto, A. (2014). Postconditioning of the small intestine: Which is the most effective algorithm in a rat model? Journal of Surgical Research, 187(2), 427-437. https://doi.org/10.1016/j.jss.2013.10.035

Postconditioning of the small intestine : Which is the most effective algorithm in a rat model? / Rosero, Oliver; Onody, Peter; Stangl, Rita; Turoczi, Zsolt; Fulop, Andras; Garbaisz, David; Lotz, G.; Harsanyi, Laszlo; Szijarto, Attila.

In: Journal of Surgical Research, Vol. 187, No. 2, 04.2014, p. 427-437.

Research output: Contribution to journalArticle

Rosero, O, Onody, P, Stangl, R, Turoczi, Z, Fulop, A, Garbaisz, D, Lotz, G, Harsanyi, L & Szijarto, A 2014, 'Postconditioning of the small intestine: Which is the most effective algorithm in a rat model?', Journal of Surgical Research, vol. 187, no. 2, pp. 427-437. https://doi.org/10.1016/j.jss.2013.10.035
Rosero, Oliver ; Onody, Peter ; Stangl, Rita ; Turoczi, Zsolt ; Fulop, Andras ; Garbaisz, David ; Lotz, G. ; Harsanyi, Laszlo ; Szijarto, Attila. / Postconditioning of the small intestine : Which is the most effective algorithm in a rat model?. In: Journal of Surgical Research. 2014 ; Vol. 187, No. 2. pp. 427-437.
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abstract = "Background Mesenteric ischemia is a serious clinical condition requiring immediate surgical intervention. The unavoidable ischemic-reperfusion (IR) injury may be ameliorated using the appropriate postconditioning protocol. The aim of the present study was to investigate the optimal postconditioning algorithm in a rat model of intestinal ischemic-reperfusion injury. Materials and methods Male Wistar rats were randomized into five groups (n = 10), one sham-operated, one IR, and three postconditioned groups, each with different protocols. The animals were subjected to 60 min of mesenteric ischemia, followed by 60 min of reperfusion. Postconditioning was applied at the onset of reperfusion using three different algorithms. Arterial pressure and mucosal microcirculation were monitored throughout the experiment. Mesenteric pH was determined at the early phase of reperfusion. Blood and tissue samples were taken at the end of reperfusion for histologic evaluation, serum lactate dehydrogenase, serum creatine kinase, serum tumor necrosis factor-α, serum interleukin-6, detailed mucosal antioxidant, and scavenger capacity assays. Results The shorter and intermediate length cycles of postconditioning enhanced mucosal microcirculation and redox state and significantly delayed the normalization of mesenteric pH. Furthermore, milder histopathologic lesions and lower concentrations of serum necroenzymes and proinflammatory cytokines were detected compared with the IR group. The protective effect of postconditioning using longer cycles could only be seen in a tendentious manner. Conclusions In a rat model of intestinal ischemia-reperfusion, the shorter and intermediate length cycles of postconditioning proved to be more effective than the use of longer cycles.",
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N2 - Background Mesenteric ischemia is a serious clinical condition requiring immediate surgical intervention. The unavoidable ischemic-reperfusion (IR) injury may be ameliorated using the appropriate postconditioning protocol. The aim of the present study was to investigate the optimal postconditioning algorithm in a rat model of intestinal ischemic-reperfusion injury. Materials and methods Male Wistar rats were randomized into five groups (n = 10), one sham-operated, one IR, and three postconditioned groups, each with different protocols. The animals were subjected to 60 min of mesenteric ischemia, followed by 60 min of reperfusion. Postconditioning was applied at the onset of reperfusion using three different algorithms. Arterial pressure and mucosal microcirculation were monitored throughout the experiment. Mesenteric pH was determined at the early phase of reperfusion. Blood and tissue samples were taken at the end of reperfusion for histologic evaluation, serum lactate dehydrogenase, serum creatine kinase, serum tumor necrosis factor-α, serum interleukin-6, detailed mucosal antioxidant, and scavenger capacity assays. Results The shorter and intermediate length cycles of postconditioning enhanced mucosal microcirculation and redox state and significantly delayed the normalization of mesenteric pH. Furthermore, milder histopathologic lesions and lower concentrations of serum necroenzymes and proinflammatory cytokines were detected compared with the IR group. The protective effect of postconditioning using longer cycles could only be seen in a tendentious manner. Conclusions In a rat model of intestinal ischemia-reperfusion, the shorter and intermediate length cycles of postconditioning proved to be more effective than the use of longer cycles.

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