Post-transcriptional inhibition of luciferase reporter assays by the nod-like receptor proteins NLRX1 and NLRC3

Arthur Ling, Fraser Soares, David O. Croitoru, Ivan Tattoli, Leticia A.M. Carneiro, Michele Boniotto, Szilvia Benko, Dana J. Philpott, Stephen E. Girardin

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22 Citations (Scopus)

Abstract

Luciferase reporter assays (LRAs) are widely used to assess the activity of specific signal transduction pathways. Although powerful, rapid and convenient, this technique can also generate artifactual results, as revealed for instance in the case of high throughput screens of inhibitory molecules. Here we demonstrate that the previously reported inhibitory effect of the Nod-like receptor (NLR) protein NLRX1 on NF-κB- and type I interferon-dependent pathways in LRAs was a nonspecific consequence of the overexpression of the NLRX1 leucine-rich repeat (LRR) domain. By comparing luciferase activity and luciferase gene expression using quantitative PCR from the same samples, we showed that NLRX1 inhibited LRAs in a post-transcriptional manner. In agreement, NLRX1 also repressed LRAs if luciferase was expressed under the control of a constitutive promoter, although the degree of inhibition by NLRX1 seemed to correlate with the dynamic inducibility of luciferase reporter constructs. Similarly, we observed that overexpression of another NLR protein, NLRC3, also resulted in artifactual inhibition of LRAs; thus suggesting that the capacity to inhibit LRAs at a post-transcriptional level is not unique to NLRX1. Finally, we demonstrate that host type I interferon response to Sendai virus infection was normal in NLRX1-silenced human HEK293T cells. Our results thus highlight the fact that LRAs are not a reliable technique to assess the inhibitory function of NLRs, and possibly other overexpressed proteins, on signal transduction pathways.

Original languageEnglish
Pages (from-to)28705-28716
Number of pages12
JournalJournal of Biological Chemistry
Volume287
Issue number34
DOIs
Publication statusPublished - Aug 17 2012

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ling, A., Soares, F., Croitoru, D. O., Tattoli, I., Carneiro, L. A. M., Boniotto, M., Benko, S., Philpott, D. J., & Girardin, S. E. (2012). Post-transcriptional inhibition of luciferase reporter assays by the nod-like receptor proteins NLRX1 and NLRC3. Journal of Biological Chemistry, 287(34), 28705-28716. https://doi.org/10.1074/jbc.M111.333146