Post-thaw storage of sperm from various salmonid species

Horváth, C. Labbé, D. Jesenšek, G. Hoitsy, G. Bernáth, D. Kaczkó, Z. Bokor, B. Urbányi

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Abstract

The effects of post-thaw storage on the motility and fertilizing capacity of cryopreserved sperm were investigated in four salmonid species, including the Adriatic grayling (Thymallus thymallus; five males), rainbow trout (Oncorhynchus mykiss; six males), marble trout (Salmo marmoratus; five males) and the brown trout (Salmo trutta m. Fario; six males). The sperm of rainbow trout and marble trout was cryopreserved in the presence of either methanol or dimethyl-sulfoxide (DMSO) as a cryoprotectant; for the sperm of the Adriatic grayling and brown trout, only methanol was used. A 1-h storage of sperm did not reduce the fertilizing capacity of the three species, whereas in the marble trout a gradual decrease in the percentage of eyed eggs in parallel with post-thaw storage time was observed. The use of methanol was found to result in higher fertilization than that of DMSO in the rainbow trout and marble trout. Remarkably high post-thaw motility expressed in % motile cells was observed following post-thaw storage of brown trout sperm for 10 (53 ± 12%) or 60 (56 ± 15%) min. The results indicate that immediate use of cryopreserved sperm for fertilization following thawing is not mandatory to attain successful fertilization.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalJournal of Applied Ichthyology
Volume31
Issue numberS1
DOIs
Publication statusPublished - Jun 1 2015

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ASJC Scopus subject areas

  • Aquatic Science

Cite this

Horváth, Labbé, C., Jesenšek, D., Hoitsy, G., Bernáth, G., Kaczkó, D., Bokor, Z., & Urbányi, B. (2015). Post-thaw storage of sperm from various salmonid species. Journal of Applied Ichthyology, 31(S1), 119-124. https://doi.org/10.1111/jai.12741