Possible role of peptides derived from otosclerotic bone in the mechanism of sensorineural hearing loss

I. Sziklai, J. Gróf, O. Ribári, J. Menyhárt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Otosclerotic stapes footplates, superstructures and temporal cortical bones were extracted with 0.25 M guanidine HO 0.5 M EDTA (pH 7.4) solution. The extracted non-collagenous peptides/proteins were separated chromatographically on a Sephadex G-25 microcolumn. The peptide composition of the bone samples were compared by capillary analytical isotachophoresis (ITP) in the molecular mass range 0.3-5 kD. The otosclerotic stapes footplate contained 13 ITP subfractions, while the stapes superstructures and cortical bone contained only 9 and 10, respectively. An otosclerosis-specific ITP subfraction was also detected in the stapes footplate, but not in the stapes superstructure or cortical bone. This subfraction was previously demonstrated in the otosclerotic perilymph as well. Four ITP subfractions occurred common in the otosclerotic stapes footplate, the superstructure and the cortical bone. Two of these common subfractions were not found in the cortical bone peptide extract, but all of them revealed higher than normal levels in the otosclerotic perilymph.

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalActa Oto-Laryngologica
Volume100
Issue number3-4
DOIs
Publication statusPublished - Jan 1 1985

Keywords

  • Isotachophoresis
  • Non-collagenous proteins and peptides

ASJC Scopus subject areas

  • Otorhinolaryngology

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