Possible role of fat tissue in the pharmacokinetics of dodeca-2E,4E,8Z,10E/ Z-tetraenoic acid isobutylamides after oral administration of Echinacea angustifolia extract in rats

Research output: Contribution to journalArticle

Abstract

Alkamides are one of the most important constituents of lipophilic extracts of Echinacea angustifolia roots. These compounds play an important role in the versatile pharmacological actions of this plant. The present study aimed to compare the concentrations of isomeric dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (DTAI) in brain and periepididymal fat tissues and blood plasma of rats. Thirty, 60, 240 and 720 min after the oral administration of E. angustifolia root extract, tissue and plasma concentrations were determined by reversed-phase HPLC with ESI-MS/MS detection. The calculated terminal t 1/2 of the mixture of DTAI was 8.28 h, which indicates a relatively slow elimination. In the 0.5-4 h period the brain/plasma and fat/plasma concentration ratios were continuously above 3 and 18, respectively, followed by equilibrium at 12 h. Our results indicate substantial accumulation of alkamides in lipid-rich tissues, which presumably contributes to a maintained pharmacological action.

Original languageEnglish
Pages (from-to)843-845
Number of pages3
JournalNatural Product Communications
Volume9
Issue number6
Publication statusPublished - 2014

Fingerprint

Echinacea angustifolia
Echinacea
oral administration
pharmacokinetics
Oral Administration
Pharmacokinetics
Fats
amides
Acids
acids
rats
extracts
lipids
brain
reversed-phase high performance liquid chromatography
Pharmacology
blood plasma
Tissue Extracts
Brain
High Pressure Liquid Chromatography

Keywords

  • Alkamide
  • Dodeca-2E,4 E,8Z,10e/Z-tetraenoic acid isobutylamides
  • Echinacea angustifolia
  • HPLC-MS/MS
  • Pharmacokinetics

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Complementary and alternative medicine
  • Plant Science

Cite this

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title = "Possible role of fat tissue in the pharmacokinetics of dodeca-2E,4E,8Z,10E/ Z-tetraenoic acid isobutylamides after oral administration of Echinacea angustifolia extract in rats",
abstract = "Alkamides are one of the most important constituents of lipophilic extracts of Echinacea angustifolia roots. These compounds play an important role in the versatile pharmacological actions of this plant. The present study aimed to compare the concentrations of isomeric dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (DTAI) in brain and periepididymal fat tissues and blood plasma of rats. Thirty, 60, 240 and 720 min after the oral administration of E. angustifolia root extract, tissue and plasma concentrations were determined by reversed-phase HPLC with ESI-MS/MS detection. The calculated terminal t 1/2 of the mixture of DTAI was 8.28 h, which indicates a relatively slow elimination. In the 0.5-4 h period the brain/plasma and fat/plasma concentration ratios were continuously above 3 and 18, respectively, followed by equilibrium at 12 h. Our results indicate substantial accumulation of alkamides in lipid-rich tissues, which presumably contributes to a maintained pharmacological action.",
keywords = "Alkamide, Dodeca-2E,4 E,8Z,10e/Z-tetraenoic acid isobutylamides, Echinacea angustifolia, HPLC-MS/MS, Pharmacokinetics",
author = "Nikoletta Jedlinszki and D. R{\'e}dei and J. Haller and T. Freund and J. Hohmann and I. Zupk{\'o}",
year = "2014",
language = "English",
volume = "9",
pages = "843--845",
journal = "Natural Product Communications",
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T1 - Possible role of fat tissue in the pharmacokinetics of dodeca-2E,4E,8Z,10E/ Z-tetraenoic acid isobutylamides after oral administration of Echinacea angustifolia extract in rats

AU - Jedlinszki, Nikoletta

AU - Rédei, D.

AU - Haller, J.

AU - Freund, T.

AU - Hohmann, J.

AU - Zupkó, I.

PY - 2014

Y1 - 2014

N2 - Alkamides are one of the most important constituents of lipophilic extracts of Echinacea angustifolia roots. These compounds play an important role in the versatile pharmacological actions of this plant. The present study aimed to compare the concentrations of isomeric dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (DTAI) in brain and periepididymal fat tissues and blood plasma of rats. Thirty, 60, 240 and 720 min after the oral administration of E. angustifolia root extract, tissue and plasma concentrations were determined by reversed-phase HPLC with ESI-MS/MS detection. The calculated terminal t 1/2 of the mixture of DTAI was 8.28 h, which indicates a relatively slow elimination. In the 0.5-4 h period the brain/plasma and fat/plasma concentration ratios were continuously above 3 and 18, respectively, followed by equilibrium at 12 h. Our results indicate substantial accumulation of alkamides in lipid-rich tissues, which presumably contributes to a maintained pharmacological action.

AB - Alkamides are one of the most important constituents of lipophilic extracts of Echinacea angustifolia roots. These compounds play an important role in the versatile pharmacological actions of this plant. The present study aimed to compare the concentrations of isomeric dodeca-2E,4E,8Z,10E/Z-tetraenoic acid isobutylamides (DTAI) in brain and periepididymal fat tissues and blood plasma of rats. Thirty, 60, 240 and 720 min after the oral administration of E. angustifolia root extract, tissue and plasma concentrations were determined by reversed-phase HPLC with ESI-MS/MS detection. The calculated terminal t 1/2 of the mixture of DTAI was 8.28 h, which indicates a relatively slow elimination. In the 0.5-4 h period the brain/plasma and fat/plasma concentration ratios were continuously above 3 and 18, respectively, followed by equilibrium at 12 h. Our results indicate substantial accumulation of alkamides in lipid-rich tissues, which presumably contributes to a maintained pharmacological action.

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